Resumen:
Since vine propagation methods can cause physiological stress, it has a great impact on sustainable viticulture. The aim of this review is to introduce the applied techniques in decreasing the time period and manage the physiological stage of vine cuttings by simultaneously manipulating the temperature of two nodes of the same cuttings in order to stimulate roots and inhibit shoot formation for vegetative propagation. To avoid mold and other bacterial diseases, antifungal and antibacterial treatment is applied. In asymptomatic situations, all cuttings should be treated with hot water before carrying out the rooting process. The cuttings are exposed to soilless aseptic hydrated media to maintain thermal isolation for emergence of root callus, at 25-27 °C during 3-4 weeks. Meanwhile, spatial temperature under 1 °C may result in chilly stress. More than 2 °C may have a significant influence on cutting performance, and above 4 °C stimulate microbial activity. Therefore, the system must maintain between 2-4 °C. This technique provides to viticulture farmers for regulation of root development and disease-free cuttings in a short time period.
