Detalle Publicación


LRRK2 haplotype-sharing analysis in Parkinson's disease reveals a novel p.S1761R mutation

Autores: Lorenzo-Betancor, O.; Samaranch Gusi, Lluís; Ezquerra, M.; Tolosa, E.; Lorenzo Ramos, María Elena; Irigoyen Irazoqui, Jaione; Gaig, C.; Pastor, María A.; Soto-Ortolaza, A.; Ross, O.; Rodríguez Oroz, María Cruz; Valldeoriola, F.; Martí, M. J.; Luquin Piudo, María Rosario Isabel; Perez-Tur, J.; Burguera, J.; Obeso Inchausti, José Ángel; Pastor Muñoz, Pau
Título de la revista: MOVEMENT DISORDERS
ISSN: 0885-3185
Volumen: 27
Número: 1
Páginas: 146 - 151
Fecha de publicación: 2012
Background and objective. Mutations in the Leucine-Rich Repeat Kinase 2 (LRRK2) gene at chromosome 12q12 are the most common genetic cause of sporadic and familial late-onset Parkinson's disease. Our aim was to identify novel LRRK2 mutations in late-onset Parkinson's disease families. Design. We analyzed chromosome 12p11.2-q13.1 haplotypes in 14 late-onset Parkinson's disease families without known LRRK2 mutations. Results. Haplotype analysis identified 12 families in which the affected subjects shared chromosome 12p11.2-q13.1 haplotypes. LRRK2 sequencing revealed a novel co-segregating missense mutation in exon 36 (c.5281A > C; p.S1761R) located within a highly conserved region of the COR [C-terminal of ROC (Ras of complex proteins)] domain wherein it could deregulate LRRK2 kinase activity by modifying ROC-COR dimer stability. p.S1761R was present in a late-onset Parkinson's disease family and in 2 unrelated Parkinson's disease subjects, but not in 2491 healthy controls. LRRK2 p.S1761R carriers developed levodopa-responsive asymmetrical parkinsonism, with variable age at onset (range: 37-72 years) suggesting age-dependent penetrance. These findings indicate that mutations interfering with LRRK2 ROC-COR domain dimerization lead to typical Parkinson's disease.