Detalle Publicación


Development of a bacterial nanoparticle vaccine

Libro: Salmonella: Methods and Protocols
Lugar de Edición: New York
Editorial: Springer New York
Fecha de publicación: 2015
Página Inicial - Final: 139 - 149
ISBN: 978-1-4939-1624-5
Resumen: A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN®).