Detalle Publicación

ARTÍCULO
Quantitative Assessment of Dietary (Poly)phenol Intake: A High-Throughput Targeted Metabolomics Method for Blood and Urine Samples
Autores: Domínguez Fernández, María Teresa; Xu, Y. ; Young Tie Yang, P. ; Alotaibi, W. ; Gibson, R. ; Hall, W. L. ; Barron, L. ; Ludwig Sanz Orrio, Iziar Amaia; Cid Canda, María Concepción; Rodriguez-Mateos, A. (Autor de correspondencia)
Título de la revista: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN: 0021-8561
Volumen: 69
Número: 1
Páginas: 537 - 554
Fecha de publicación: 2021
Resumen:
Many studies have associated the consumption of (poly)phenol-rich diets with health benefits. However, accurate high-throughput quantitative methods for estimating exposure covering a broad spectrum of (poly)phenols are lacking. We have developed and validated a high-throughput method for the simultaneous quantification of 119 (poly)phenol metabolites in plasma and urine using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry, with a very fast sample treatment and a single run time of 16 min. This method is highly sensitive, precise, accurate, and shows good linearity for all compounds (R2 > 0.992). This novel method will allow a quantitative assessment of habitual (poly)phenol intake in large epidemiological studies as well as clinical studies investigating the health benefits of dietary (poly)phenols.