Solution blow spinning, SBS, was used to prepare fibrous films of thermoplastic nanocomposites with potential antibacterial properties based on polysulfone, PSF, filled with well dispersed TiO2 nanoparticles. The PSF/TiO2 nanocomposites were produced with different nanoparticles content up to 10% by weight. A wide characterization was carried out focusing on the morphology at the nanoscale, roughness, contact angles, and surface free energy. Cell adhesion was studied by inspection by scanning electron microscopy (SEM). A uniform dispersion of the nanofiller was achieved, with the nanoparticles evenly embedded in the polymer along the fibers when they were created during the blow spinning process. TiO2 content influenced the topography of the films, most likely due to a direct effect on the solvent evaporation rate. The results obtained pointed out that an increase of the surface hydrophobicity as a result of the increased roughness induced by the presence of TiO2 nanoparticles was the main contribution to the reduction of DH5¿ Escherichia coli cells adhesion.