Detalle Publicación

ARTÍCULO
Heterogeneity in allergy to mollusks: a clinical-immunological study in a population from the north of Spain
Autores: Azofra, J.; Echechipia, S.; Irazábal, B.; Muñoz, D.; Bernedo, N.; García, B.E.; Gastaminza Lasarte, Gabriel; Goikoetxea Lapresa, María José; Joral, A.; Lasa, E.; Gamboa, P.M.; Díaz, C.; Beristáin, A.; Quiñones, D.; Bernaola, G.; Echenagusia, M.A.; Liarte, I.; García, E.; Cuesta, J.; Martínez, M.D.; Velasco, M.; Longo, N.; Pastor-Vargas, C.
Título de la revista: JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
ISSN: 1018-9068
Volumen: 27
Número: 4
Páginas: 252 - 260
Fecha de publicación: 2017
Lugar: WOS
Resumen:
Background: Allergy to mollusks has been the focus of fewer studies than allergy to crustaceans. Furthermore, allergy to mollusks is less well characterized. Objectives: To describe the clinical characteristics of mollusk-allergic patients, to identify the responsible allergens, and to assess cross reactivity. Methods: We performed a prospective multicenter study including 45 patients with mollusk allergy, which was diagnosed based on a suggestive clinical history and a positive skin test result with the agent involved. Fractions were identified using SDS-PAGE and immunoblotting. The proteins responsible were subsequently identified using mass spectrometry. ELISA inhibition studies were performed with mollusks, dust mites, and crustaceans. Results: We found that 25 patients (55%) were allergic to cephalopods, 14 (31%) to bivalves, and 11 (24%) to gastropods. Limpet was the third most frequent cause of allergy (15% of cases). In 31 patients (69%), the manifestation was systemic; 10 (22%) exhibited oral allergy syndrome, and 7 (15%) experienced contact urticaria. Most major allergens were found between 27 kDa and 47 kDa. ELISA inhibition assays revealed a high degree of inhibition of cephalopods and bivalves from all the groups of mollusks, mites, and crustaceans. Mass spectrometry identified tropomyosin, actin, and myosin as the major allergens. Conclusions: Cephalopods, especially squid, are the mollusks that most frequently trigger allergic symptoms. The very frequent occurr