Detalle Publicación

ARTÍCULO
Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells
Título de la revista: STEM CELL RESEARCH
ISSN: 1873-5061
Volumen: 21
Páginas: 40-43
Fecha de publicación: 2017
Resumen:
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.