Detalle Publicación


Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression

Autores: Belicha-Villanueva, A.; Rodríguez Madoz, Juan Roberto; Maamary, J.; Baum, A.; Bernal-Rubio, D.; Minguito de la Escalera, M.; Fernandez-Sesma, A.; García-Sastre, A.
Título de la revista: JOURNAL OF VIROLOGY
ISSN: 0022-538X
Volumen: 86
Número: 10
Páginas: 5926 - 5930
Fecha de publicación: 2012
Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.