Detalle Publicación

T Cell Migration from inflamed skin to draining lymph nodes requires intralymphatic crawling supported by ICAM-1/LFA-1 interactions.
Autores: Teijeira Sánchez, Álvaro; Hunter, M. C.; Russo, E.; Proulx, S. T.; Frei, T.; Debes, G. F.; Coles, M.; Melero Bermejo, Ignacio; Detmar, M.; Rouzaut Subirá, Ana; Halin, C.
Título de la revista: CELL REPORTS
ISSN: 2211-1247
Volumen: 18
Número: 4
Páginas: 857 - 865
Fecha de publicación: 2017
T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood. Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels. Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants. In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade. Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.