Detalle Publicación

ARTÍCULO
Interactions of poly (anhydride) nanoparticles with macrophages in light of their vaccine adjuvant propertieS
Título de la revista: INTERNATIONAL JOURNAL OF PHARMACEUTICS
ISSN: 0378-5173
Volumen: 496
Número: 2
Páginas: 922 - 930
Fecha de publicación: 2015
Resumen:
Understanding how nanoparticles are formed and how those processes ultimately determine the nanoparticles¿ properties and their impact on their capture by immune cells is key in vaccination studies. Accordingly, we wanted to evaluate how the previously described poly (anhydride)-based nanoparticles of the copolymer of methyl vinyl ether and maleic anhydride (NP) interact with macrophages, and how this process depends on the physicochemical properties derived from the method of preparation. First, we studied the influence of the desolvation and drying processes used to obtain the nanoparticles. NP prepared by the desolvation of the polymers in acetone with a mixture of ethanol and water yielded higher mean diameters than those obtained in the presence of water (250 nm vs. 180 nm). In addition, nanoparticles dried by lyophilization presented higher negative zeta potentials than those dried by spray-drying (¿47 mV vs. ¿35 mV). Second, the influence of the NP formulation on the phagocytosis by J774 murine macrophage-like cell line was investigated. The data indicated that NPs prepared in the presence of water were at least three-times more efficiently internalized by cells than NPs prepared with the mixture of ethanol and water. Besides, lyophilized nanoparticles appeared to be more efficiently taken up by J744 cells than those dried by spray-drying. To further understand the specific mechanisms involved in the cellular internalization of NPs, different pharmacological inhibitors