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ARTÍCULO

Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

Autores: Coppiello, Giulia; Abizanda Sarasa, Gloria María; Aguado, N.; Iglesias López, Elena; Iglesias García, Olalla; Lo Nigro, A.; Prosper Cardoso, Felipe; Aranguren López, Xabier
Título de la revista: STEM CELL RESEARCH
ISSN: 1873-5061
Volumen: 21
Páginas: 40-43
Fecha de publicación: 2017
Resumen:
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.
DOI: https://doi.org/10.1016/j.scr.2017.03.020.
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