Detalle Publicación


Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment

Autores: Celay Leoz, Ion; Blanco, I.; Lázcoz Ripoll, Paula; Rotinen, M.; Sáez Castresana, Javier; Encío, I.
Título de la revista: PLOS ONE
ISSN: 1932-6203
Volumen: 8
Número: 5
Páginas: e62771
Fecha de publicación: 2013
To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.