Detalle Publicación

ARTÍCULO
3D reconstruction of histological sections: Application to mammary gland tissue
Título de la revista: MICROSCOPY RESEARCH AND TECHNIQUE
ISSN: 1059-910X
Volumen: 73
Número: 11
Páginas: 1019-1029
Fecha de publicación: 2010
Resumen:
In this article, we present a novel method for the automatic 3D reconstruction of thick tissue blocks from 2D histological sections. The algorithm completes a high-content (multiscale, multifeature) imaging system for simultaneous morphological and molecular analysis of thick tissue samples. This computer-based system integrates image acquisition, annotation, registration, and three-dimensional reconstruction. We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high-resolution molecular data.