Detalle Publicación


Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells

Autores: Rice, C. M.; Clavel, C.; Mazo Vega, Manuel María; Prosper Cardoso, Felipe; Scolding, N. J.
Título de la revista: British Journal of Haematology
ISSN: 0007-1048
Volumen: 148
Número: 3
Páginas: 441 - 444
Fecha de publicación: 2010
This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.