Detalle Publicación

ARTÍCULO
Development and validation of a HPLC method for the determination of cyclosporine a in new bioadhesive nanoparticles for oral Administration
Título de la revista: INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN: 0250-474X
Volumen: 76
Número: 2
Páginas: 132 - 137
Fecha de publicación: 2014
Resumen:
A simple and reliable high performance liquid chromatography method was developed and validated for the rapid determination of cyclosporine A in new pharmaceutical dosage forms based on the use of poly (methylvinylether¿co¿maleic anhydride) nanoparticles. The chromatographic separation was achieved using Ultrabase C18 column (250×4.6 mm, 5 ¿m), which was kept at 75°. The gradient mobile phase consisted of acetonitrile and water with a flow rate of 1 ml/min. The effluent was monitored at 205 nm using diode array detector. The method exhibited linearity over the assayed concentration range (22¿250 ¿g/ml) and demonstrated good intraday and interday precision and accuracy (relative standard deviations were less than 6.5% and the deviation from theoretical values is below 5.5%). The detection limit was 1.36 ¿g/ml. This method was also applied for quantitative analysis of cyclosporine A released from poly (methylvinylether¿co¿maleic anhydride) nanoparticles. Key words: Cyclosporine A, HPLC¿UV, nanoparticles, poly (methylvinylether¿co¿maleic anhydride), oral administration