Detalle Publicación

ARTÍCULO
The lipopolysaccharide core of Brucella abortus acts as a shield against innate immunity recognition
Autores: Conde Álvarez, Raquel; Arce - Gorvel, V.; Iriarte Cilveti, Maite; Mancek-Kleber, M.; Barquero Calvo, E.; Palacios Chaves, Leyre; Chacón Díaz, C.; Chaves Olarte, E.; Martirosyan, A.; von Bargen, K.; Grilló, M. J.; Jerala, R.; Brandenburg, K.; Llobet, E.; Bengoechea, J. A.; Moreno, E.; Moriyón Uría, Ignacio; Gorvel, J. P.
Título de la revista: PLOS PATHOGENS
ISSN: 1553-7374
ISBN: 1553-7366
Volumen: 8
Número: 5
Páginas: e1002675
Fecha de publicación: 2012
Resumen:
Innate immunity recognizes bacterial molecules bearing pathogen-associated molecular patterns to launch inflammatory responses leading to the activation of adaptive immunity. However, the lipopolysaccharide (LPS) of the gram-negative bacterium Brucella lacks a marked pathogen-associated molecular pattern, and it has been postulated that this delays the development of immunity, creating a gap that is critical for the bacterium to reach the intracellular replicative niche. We found that a B. abortus mutant in the wadC gene displayed a disrupted LPS core while keeping both the LPS O-polysaccharide and lipid A. In mice, the wadC mutant induced proinflammatory responses and was attenuated. In addition, it was sensitive to killing by non-immune serum and bactericidal peptides and did not multiply in dendritic cells being targeted to lysosomal compartments. In contrast to wild type B. abortus, the wadC mutant induced dendritic cell maturation and secretion of pro-inflammatory cytokines. All these properties were reproduced by the wadC mutant purified LPS in a TLR4-dependent manner. Moreover, the core-mutated LPS displayed an increased binding to MD-2, the TLR4 co-receptor leading to subsequent increase in intracellular signaling. Here we show that Brucella escapes recognition in early stages of infection by expressing a shield against recognition by innate immunity in its LPS core and identify a novel virulence mechanism in intracellular pathogenic gram-negative bacteria. These results also encourage for an improvement in the generation of novel bacterial vaccines.