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ARTÍCULO

Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors

Autores: Lane-Reticker, S. K.; Kessler, E. A.; Muscato, A. J. ; Kim, S. Y. ; Doench, J. G.; Yates, K. B.; Manguso, R. T. (Autor de correspondencia); Dubrot Armendáriz, Juan (Autor de correspondencia)
Título de la revista: STAR PROTOCOLS
ISSN: 2666-1667
Volumen: 4
Número: 1
Páginas: 102082
Fecha de publicación: 2023
Resumen:
Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in Renca mouse model. This protocol describes how to conduct an in vivo genetic screen with a sgRNA library and SCAR vectors that can be applied to different cell lines and contexts.For complete details on the use and execution of this protocol, please refer to Dubrot et al. (2021).1
DOI: https://doi.org/10.1016/j.xpro.2023.102082
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