Detalle Publicación


Unraveling the diagnosis of kiwifruit allergy: usefulness of current diagnostic tests

Autores: D Amelio Garofalo, Carmen Mariana; Bernad Alonso, Amalia (Autor de correspondencia); García-Figueroa, B. E.; Garrido-Fernández, S.; Azofra, J.; Beristain, A.; Bueno-Díaz, C.; Garrido-Arandia, M.; Gastaminza Lasarte, Gabriel; Ferrer Puga, Marta; Díaz Perales, A.; Villalba, M.; Goikoetxea Lapresa, María José
ISSN: 1018-9068
Volumen: 32
Número: 3
Páginas: 206 - 212
Fecha de publicación: 2022
Objectives: To determine the usefulness of the in vitro and in vivo methods used in the diagnosis of kiwifruit allergy and to specifically assess the impact of seed proteins on sensitivity. Methods: We performed skin prick tests (SPTs) using various commercial extracts, homemade pulp, and seed extracts and prick-prick tests with kiwifruit on 36 allergic patients. The presence of specific IgE (sIgE) was assessed using the ImmunoCAP (kiwifruit extract), ELISA (Act d 1, Act d 2), ISAC, and FABER assays. Immunoblotting of seed extract was carried out, and a single-blind oral food challenge was performed with whole seeds in seed-sensitized individuals. Results: The prick prick test with kiwifruit demonstrated the highest diagnostic capacity (81.8% sensitivity and 94.1% specificity) among the in vivo tests. The sIgE levels measured using ImmunoCAP (kiwifruit extract) showed a similar sensitivity to that of global ISAC and FABER (63.9%, 59.5%, and 58.3%, respectively). Act d 1 was the major allergen. Sensitization to Act d 1 was associated with positive sIgE results to whole kiwifruit extract detected by ImmunoCAP (P<.000). A positive SPT result to kiwifruit seeds was associated with severe symptoms induced by kiwifruit (P=.019) as a marker of advanced disease, but not with clinically relevant sensitization. Challenge testing with kiwifruit seeds performed on 8 seed-sensitized patients yielded negative results. Conclusions: Sensitization to Act d 1 is associated with a positive result in conventional diagnostic techniques, whereas kiwifruit seed sensitization does not increase the sensitivity of the diagnostic techniques evaluated.