Detalle Publicación

ARTÍCULO
PPARGC1A Gene Promoter Methylation as a Biomarker of Insulin Secretion and Sensitivity in Response to Glucose Challenges
Autores: Santos, J. L. (Autor de correspondencia); Krause, B. J. ; Cataldo, L. R.; Vega, J. ; Salas-Perez, F. ; Mennickent, P. ; Gallegos, R.; Milagro Yoldi, Fermín Ignacio; Prieto Hontoria, Pedro Luis; Riezu Boj, José Ignacio; Bravo, C.; Salas-Huetos, A. ; Arpon Miranda, Ana; Galgani, J. E. ; Martínez Hernández, Alfredo
Título de la revista: NUTRIENTS
ISSN: 2072-6643
Volumen: 12
Número: 9
Páginas: 2790
Fecha de publicación: 2020
Lugar: WOS
Resumen:
Methylation in CpG sites of the PPARGC1A gene (encoding PGC1-alpha) has been associated with adiposity, insulin secretion/sensitivity indexes and type 2 diabetes. We assessed the association between the methylation profile of the PPARGC1A gene promoter gene in leukocytes with insulin secretion/sensitivity indexes in normoglycemic women. A standard oral glucose tolerance test (OGTT) and an abbreviated version of the intravenous glucose tolerance test (IVGTT) were carried out in n = 57 Chilean nondiabetic women with measurements of plasma glucose, insulin, and C-peptide. Bisulfite-treated DNA from leukocytes was evaluated for methylation levels in six CpG sites of the proximal promoter of the PPARGC1A gene by pyrosequencing (positions -816, -783, -652, -617, -521 and -515). A strong correlation between the DNA methylation percentage of different CpG sites of the PPARGC1A promoter in leukocytes was found, suggesting an integrated epigenetic control of this region. We found a positive association between the methylation levels of the CpG site -783 with the insulin sensitivity Matsuda composite index (rho = 0.31; p = 0.02) derived from the OGTT. The CpG hypomethylation in the promoter position -783 of the PPARGC1A gene in leukocytes may represent a biomarker of reduced insulin sensitivity after the ingestion of glucose.