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ARTÍCULO

Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

Autores: Consuegra, I.; Rodriguez-Aierbe, C. ; Santiuste, I. ; Bosch, A. ; Martinez-Marin, R. ; Fortuño Cebamanos, María Antonia; Diaz, T. ; Marti, S. ; Munoz Fernandez, M. A.
Título de la revista: BIOPRESERVATION AND BIOBANKING
ISSN: 1947-5535
Volumen: 15
Número: 4
Páginas: 305 - 309
Fecha de publicación: 2017
Resumen:
The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.
DOI: https://doi.org/10.1089/bio.2016.0105
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