Detalle Publicación


PDGFR alpha(+) Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors

Autores: Lo Nigro, A.; de Jaime-Soguero, A.; Khoueiry, R.; Cho, D. S. ; Ferlazzo, G. M.; Perini, I.; Escalona, V. A.; Aranguren López, Xabier; Lopes, S. M. C. D.; Koh, K. P.; Conaldi, P. G.; Hu, W. S. ; Zwijsen, A. ; Lluis, F. ; Verfaillie, C. M.
Título de la revista: STEM CELL REPORTS
ISSN: 2213-6711
Volumen: 8
Número: 2
Páginas: 318 - 333
Fecha de publicación: 2017
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFR alpha) positive. Here, we demonstrated that cultured mouse embryonic stem cells (mESCs) express PDGFRa heterogeneously, fluctuating between a PDGFR alpha+ (PrE-primed) and a platelet endothelial cell adhesion molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third subpopulation, expressing epiblast and PrE determinants (double-positive). In vitro, these subpopulations differ in their self-renewal and differentiation capability, transcriptional and epigenetic states. In vivo, double- positive cells contributed to epiblast and PrE, while PrE-primed cells exclusively contributed to PrE derivatives. The transcriptome of PDGFR alpha(+) subpopulations differs from previously described subpopulations and shows similarities with early/ mid blastocyst cells. The heterogeneity did not depend on PDGFRa but on leukemia inhibitory factor and fibroblast growth factor signaling and DNA methylation. Thus, PDGFR alpha(+) cells represent the in vitro counterpart of in vivo PrE precursors, and their selection from cultured mESCs yields pure PrE precursors.