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Implementation and characterization of a fully miniaturized biosensor for endotoxin detection based on electrochemical techniques

Título de la revista: IEEE SENSORS JOURNAL
ISSN: 1530-437X
Volumen: 14
Número: 1
Páginas: 270 - 277
Fecha de publicación: 2014
In this paper, the implementation and characterization of a hand-held and simple biosensor for in-situ endotoxin determination are described. The integrated biosensor developed here is based on the electrochemical detection of endotoxin using polymyxin B as bioreceptor immobilized onto gold electrodes via a self-assembled monolayer. The cyclic voltammetry, electrochemical impedance spectroscopy, and atomic force microscopy were used to characterize the biosensor performance and properties throughout the functionalization process. In addition, a comparative analysis of the behavior and features of two alternative electrochemical techniques for endotoxin detection was carried out. The biosensing device fabricated by thin-film technologies provided a simple and robust method to detect low concentrations of endotoxin.