Detalle Publicación

A multimodalsScaffold for SDF1 delivery improves cardiac function in a rat subacute myocardial infarct model

Autores: Pérez Estenaga, Íñigo; Chevalier, M. T.; Pena, E.; Abizanda Sarasa, Gloria María; Alsharabasy, A. M.; Larequi Ardanáz, Eduardo; Cilla, M.; Perez, M. M.; Gurtubay, J.; García de Yébenes Castro, Manuel; Prosper Cardoso, Felipe; Pandit, A. (Autor de correspondencia); Pelacho Samper, Beatriz (Autor de correspondencia)
ISSN: 1944-8244
Volumen: 15
Número: 44
Páginas: 50638 - 50651
Fecha de publicación: 2023
Ischemic heart diseaseis one of the leading causes ofdeath worldwide.The efficient delivery of therapeutic growth factors could counteractthe adverse prognosis of post-myocardial infarction (post-MI). Inthis study, a collagen hydrogel that is able to load and appropriatelydeliver pro-angiogenic stromal cell-derived factor 1 (SDF1) was physicallycoupled with a compact collagen membrane in order to provide the suturestrength required for surgical implantation. This bilayer collagen-on-collagenscaffold (bCS) showed the suitable physicochemical properties thatare needed for efficient implantation, and the scaffold was able todeliver therapeutic growth factors after MI. In vitro collagen matrix biodegradation led to a sustained SDF1 release anda lack of cytotoxicity in the relevant cell cultures. In vivo intervention in a rat subacute MI model resulted in the full integrationof the scaffold into the heart after implantation and biocompatibilitywith the tissue, with a prevalence of anti-inflammatory and pro-angiogenicmacrophages, as well as evidence of revascularization and improvedcardiac function after 60 days. Moreover, the beneficial effect ofthe released SDF1 on heart remodeling was confirmed by a significantreduction in cardiac tissue stiffness. Our findings demonstrate thatthis multimodal scaffold is a desirable matrix that can be used asa drug delivery system and a scaffolding material to promote functionalrecovery after MI.