Detalle Publicación

Simple and practical multigram synthesis of D-xylonate using a recombinant xylose dehydrogenase

Autores: Sanchez-Moreno, I. (Autor de correspondencia); Benito-Arenas, R.; Montero Calle, Maria del Pilar; Hermida, C.; Garcia-Junceda, E. ; Fernandez-Mayoralas, A.
Título de la revista: ACS OMEGA
ISSN: 2470-1343
Volumen: 4
Número: 6
Páginas: 10593 - 10598
Fecha de publicación: 2019
An efficient multienzyme system for the preparatiy e synthesis of D-xylonate, a chemical with versatile industrial applications, is described. The multienzyme system is based on D-xylose oxidation catalyzed by the xylose dehydrogenase from Calulobacter crescentus and the use of catalytic amounts of NAD(+). The cofactor is regenerated in situ by coupling the reduction of acetaldehyde into ethanol catalyzed by alcohol dehydrogenase from Clostridium kluyveri. Excellent conversions (>95%) were obtained in a process that allows easy product isolation by simple evaporation of the volatile buffer and byproducts.