Detalle Publicación

PR-LncRNA signature regulates glioma cell activity through expression of SOX factors

Autores: Torres-Bayona, S.; Aldaz, P.; Auzmendi-Iriarte, J.; Saenz-Antonanzas, A. ; Garcia, I.; Arrazola, M.; Gerovska, D.; Undabeitia, J. ; Querejeta, A. ; Egana, L.; Villanua, J.; Ruiz, I. ; Sarasqueta, C. ; Urculo, E.; Arauzo-Bravo, M. J. ; Huarte Martínez, Maite; Sampron, N.; Matheu, A. (Autor de correspondencia)
Título de la revista: SCIENTIFIC REPORTS
ISSN: 2045-2322
Volumen: 8
Número: 12746
Fecha de publicación: 2018
Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.