Nuestros investigadores

Ana Cristina Villaro Gumpert

Publicaciones científicas más recientes (desde 2010)

Autores: Gil Iturbe, Eva; Castilla Madrigal, Rosa María; Barreneche Huici, Jayone; et al.
ISSN 0021-9541  Vol. 234  Nº 4  2019  págs. 4396-4408
Autores: Gil Iturbe, Eva; Castilla Madrigal, Rosa María; Barrenetxe, J.; et al.
ISSN 1742-464X  Vol. 284  Nº Supl. 1  2017  págs. 157 - 158
Autores: Redrado Jordán, Miriam; Bodegas Frías, María Elena; Villaro Gumpert, Ana Cristina; et al.
ISSN 0213-3911  Vol. 28  Nº 8  2013  págs. 1029 - 1040
Inhibitor of differentiation-1 (Id1) plays a role in cell proliferation, acquisition of epithelial to mesenchymal transition (EMT) features and angiogenesis. Id1 was shown to be expressed in some tumor types, mainly in advanced dedifferentiated stages. However, recent studies using a validated and highly specific monoclonal antibody against Id1 have challenged many of the results obtained by immunohistochemistry. The goal of our work was to perform a thorough analysis of Id1 expression in mouse embryos and adult tissues, as well as healthy and malignant mouse and human samples using this validated antibody (Perk et al., 2006). Our results show that Id1 was highly expressed in the oropharyngeal cavity, lung, cartilage and skin of E14 and E15 mouse embryos, but expression was progressively reduced in more developed embryos. Immunostaining only remained in epithelial cells of the gut and uterus of adult mice. Mammary MMTV-Myc and MMTV-Myc/VEGF transgenic mouse tumors, and squamous cell carcinomas of the lung induced by N-nitroso-tris-chloroethylurea (NTCU) were highly positive for Id1, unlike their respective healthy counterparts. Id1 immunostaining in a human tissue microarray (TMA) revealed strong expression in cancers of the oral cavity, bladder and cervix. Some tumor specimens of esophagus, thyroid and breast were also strongly positive. Our results suggest that Id1 is an oncofetal protein highly expressed in particular tumor types that should be reanalyzed in future studies using large cohorts of patients to reassess its diagnostic/prognostic value. Moreover, MMTV-Myc- and NTCU-induced tumors could serve as appropriate mouse models to study Id1 functions in breast and lung cancer, respectively.