ARTÍCULO

A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues

Autores: Tamames Tabar, Cristina; Imbuluzqueta Iturburua, Edurne; Campanero Martínez, Miguel Ángel; Horcajada, P.; Blanco Prieto, María José
Título de la revista: JOURNAL OF CHROMATOGRAPHY. B, BIOMEDICAL SCIENCES AND APPLICATIONS
ISSN: 1387-2273
Volumen: 935
Páginas: 47 - 53
Fecha de publicación: 2013
Resumen:
A simple liquid-liquid extraction procedure and quantification by high-performance liquid chromatography (HPLC) method coupled to a diode-array detector (DAD) of genistein (GEN) was developed in various mouse biological matrices. 7-ethoxycoumarin was used as internal standard (IS) and peaks were optimally separated using a Kinetex C18 column (2.6 mu m. 150 mm x 2.10 mm I.D.) at 40 degrees C with an isocratic elution of mobile phase with sodium dihydrogen phosphate 0.01 M in water at pH 2.5 and methanol (55:45, v/v), at a flow rate of 0.25 mL/min. The injection volume was 10 mu L. In all cases, the range of GEN recovery was higher than 61%. The low limit of quantification (LLOQ) was 25 ng/mL. The linearity of the calibration curves was satisfactory in all cases as shown by correlation coefficients >0.996. The within-day and between-day precisions were <15% and the accuracy ranged in all cases between 90.14% and 106.05%. This method was successfully applied to quantify GEN in liver, spleen, kidney and plasma after intravenous administration of a single dose (30 mg/kg) in female BALB/C mice