Production of highly pure human glycosylated GDNF in a mammalian cell line

Autores: Ansorena Artieda, Eduardo; Garbayo Atienza, Elisa; Lanciego Pérez, José Luis; Aymerich Soler, María Soledad; Blanco Prieto, María José
ISSN: 0378-5173
Volumen: 385
Número: 1-2
Páginas: 6 - 11
Fecha de publicación: 2010
The administration of glial cell line-derived neurotrophic factor (GDNF) has emerged as a promising strategy for the treatment of several diseases of the nervous system as Parkinson's disease, amyotrophic lateral sclerosis, spinal cord injury and nerve regeneration as well as ocular diseases and drug addictions. A procedure for the purification of human recombinant glycosylated GDNF using a mammalian expression system as the source of the protein is discussed in the present paper. The neurotrophic factor was purified using cation exchange chromatography and gel filtration. A human cell line was chosen as the source of therapeutic protein, since a recombinant protein with a structure and glycosylation pattern equivalent to the native form is desirable for its prospective therapeutic utilization. The activity of the highly pure protein obtained was confirmed with a cell-based bioassay. The purified protein is suitable for its in vivo evaluation in animals and for possible subsequent clinical application.