ARTÍCULO

Helichrysum and grapefruit extracts inhibit carbohydrate digestion and absorption, improving postprandial glucose levels and hyperinsulinemia in rats

Autores: de la Garza Hernández, Ana Laura Isabel; Etxeberría Aramburu, Usune; Lostao Crespo, María del Pilar; San Román Aberasturi, Beatriz; Barreneche Huici, Jayone; Martínez Hernández, Alfredo; Milagro Yoldi, Fermín Ignacio
Título de la revista: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN: 0021-8561
Volumen: 61
Número: 49
Páginas: 12012 - 12019
Fecha de publicación: 2013
Resumen:
Several plant extracts rich in flavonoids have been reported to improve hyperglycemia by inhibiting digestive enzyme activities and SGLT1-mediated glucose uptake. In this study, helichrysum ( Helichrysum italicum ) and grapefruit ( Citrus × paradisi ) extracts inhibited in vitro enzyme activities. The helichrysum extract showed higher inhibitory activity of ¿-glucosidase (IC50 = 0.19 mg/mL) than ¿-amylase (IC50 = 0.83 mg/mL), whereas the grapefruit extract presented similar ¿-amylase and ¿-glucosidase inhibitory activities (IC50 = 0.42 mg/mL and IC50 = 0.41 mg/mL, respectively). Both extracts reduced maltose digestion in noneverted intestinal sacs (57% with helichrysum and 46% with grapefruit). Likewise, both extracts inhibited SGLT1-mediated methylglucoside uptake in Caco-2 cells in the presence of Na+ (56% of inhibition with helichrysum and 54% with grapefruit). In vivo studies demonstrated that helichrysum decreased blood glucose levels after an oral maltose tolerance test (OMTT), and both extracts reduced postprandial glucose levels after the oral starch tolerance test (OSTT). Finally, both extracts improved hyperinsulinemia (31% with helichrysum and 50% with grapefruit) and HOMA index (47% with helichrysum and 54% with grapefruit) in a dietary model of insulin resistance in rats. In summary, helichrysum and grapefruit extracts improve postprandial glycemic control in rats, possibly by inhibiting ¿-glucosidase and ¿-amylase enzyme activities and decreasing SGLT1-mediated glucose uptake.