ARTÍCULO

Anti-proliferative effect of melissa officinalis on human colon cancer cell line

Autores: Encalada, M.A.; Hoyos, K.M.; Rehecho Pérez, Sheyla; Berasategi Zabalza, Izaskun; García Íñiguez de Ciriano, Mikel; Ansorena Artieda, Diana María; Astiasarán Anchía, María Icíar; Navarro Blasco, Íñigo; Cavero Remón, Rita Yolanda; Calvo Martínez, María Isabel
Título de la revista: Plant foods for human nutrition
ISSN: 0921-9668
Volumen: 66
Número: 4
Páginas: 328 - 334
Fecha de publicación: 2011
Resumen:
Melissa officinalis L. (Lamiaceae) is consumed as a traditional herbal tea in the Mediterranean region. The cytotoxic effect of the 50% ethanolic and aqueous extract, determined by the MTT and NR assays, was evaluated in vitro on Human Colon Cancer Cell Line (HCT-116), using Triton 10% as positive control. The 50% ethanolic extract showed significant differences after 72 h of treatment, reducing cell proliferation to values close to 40%, even the lowest dose tested (5 mu g/ml). In the MTT assay, the same extract caused the lowest cell viability with 13% at a concentration of 1,000 mu g/ml after 72 h of treatment, being a value lower than Triton 10%. The antioxidant activity was also confirmed evaluating the capacity of the extracts to scavenge ABTS and DPPH radicals, and IC(50) values were highly correlated with the total phenolic and flavonoid content. Bioassay guided fractionation led to the isolation of an anti-proliferative compound, rosmarinic acid. Its structural elucidation was performed by HPLC/DAD/ESI/MS analysis. High dose of rosmarinic acid (1,000 mu g/ml) was clearly cytotoxic against HCT-116 cells, with a significant decrease in cell number since the earliest time point (24 h).