Detalle Publicación

The urinary transcriptome as a source of biomarkers for prostate cancer

Autores: Solé, C.; Goicoechea Oroz, Ibai; Goñi, A.; Schramm, M.; Armesto, M.; Arestín, M.; Manterola, L.; Tellaetxe, M.; Alberdi, A.; Nogueira, L.; Roumiguié, M.; López, J. I.; Jaka, J. P. S.; Urruticoechea, A.; Vergara, I.; Loizaga-Iriarte, A.; Unda, M.; Carracedo, A.; Malavaud, B.; Lawrie, C. H. (Autor de correspondencia)
Título de la revista: CANCERS
ISSN: 2072-6694
Volumen: 12
Número: 2
Páginas: 513
Fecha de publicación: 2020
Prostate cancer (PCa) is the second most common cancer of men and is typically slow-growing and asymptomatic. The use of blood PSA as a screening method has greatly improved PCa diagnosis, but high levels of false positives has raised much interest in alternative biomarkers. We used next-generation sequencing (NGS) to elucidate the urinary transcriptome of whole urine collected from high-stage and low-stage PCa patients as well as from patients with the confounding diagnosis of benign hyperplasia (BPH). We identified and validated five differentially expressed protein-coding genes (FTH1 BRPF1, OSBP, PHC3, and UACA) in an independent validation cohort of small-volume (1 mL) centrifuged urine (n = 94) and non-centrifuged urine (n = 84) by droplet digital (dd)PCR. These biomarkers were able to discriminate between BPH and PCa patients and healthy controls using either centrifuged or non-centrifuged whole urine samples, suggesting that the urinary transcriptome is a valuable source of non-invasive biomarkers for PCa that warrants further investigation.