Iron-dependent reset of the flower transcriptome, proteome, metabolome and hormonal content in an Arabidopsis ferritin mutant

Autores: Sudre, D.; Gutierrez-Carbonell, E.; Lattanzio, G.; Rellán-Álvarez, R.; Gaymard, F.; Fiehn, O.; Álvarez-Fernández, A.; Zamarreño Arregui, Ángel; Bacaicoa, E.; Duy, D.; García-Mina Freire, José María; Abadía, J.; Philippar, K.; López-Millán, A. F.; Briat, J. F. (Autor de correspondencia)
ISSN: 0022-0957
Volumen: 64
Número: 10
Páginas: 2665 - 2668
Fecha de publicación: 2013
Iron homeostasis is an important process for flower development and plant fertility. The role of plastids in these processes has been shown to be essential. To document the relationships between plastid iron homeostasis and flower biology further, a global study (transcriptome, proteome, metabolome, and hormone analysis) was performed of Arabidopsis flowers from wild-type and triple atfer1-3-4 ferritin mutant plants grown under iron-sufficient or excess conditions. Some major modifications in specific functional categories were consistently observed at these three omic levels, although no significant overlaps of specific transcripts and proteins were detected. These modifications concerned redox reactions and oxidative stress, as well as amino acid and protein catabolism, this latter point being exemplified by an almost 10-fold increase in urea concentration of atfer1-3-4 flowers from plants grown under iron excess conditions. The mutant background caused alterations in Fe-haem redox proteins located in membranes and in hormone-responsive proteins. Specific effects of excess Fe in the mutant included further changes in these categories, supporting the idea that the mutant is facing a more intense Fe/redox stress than the wild type. The mutation and/or excess Fe had a strong impact at the membrane level, as denoted by the changes in the transporter and lipid metabolism categories.