Adherence to Mediterranean diet is associated with methylation changes in inflammation-related genes in peripheral blood cells

Autores: Arpón, A.; Riezu Boj, José Ignacio; Milagro Yoldi, Fermín Ignacio; Martí del Moral, Amelia; Razquin Burillo, Cristina; Martínez González, Miguel Ángel; Corella, D.; Estruch, R.; Casas, R.; Fitó, M.; Ros, E.; Salas-Salvadó, J.; Martínez Hernández, Alfredo
ISSN: 1138-7548
Volumen: 73
Número: 3
Páginas: 445 - 455
Fecha de publicación: 2017
Epigenetic processes, including DNA methylation, might be modulated by environmental factors such as the diet, which in turn have been associated with the onset of several diseases such as obesity or cardiovascular events. Meanwhile, Mediterranean diet (MedDiet) has demonstrated favourable effects on cardiovascular risk, blood pressure, inflammation and other complications related to excessive adiposity. Some of these effects could be mediated by epigenetic modifications. Therefore, the objective of this study was to investigate whether the adherence to MedDiet is associated with changes in the methylation status from peripheral blood cells. A subset of 36 individuals was selected within the Prevencion con Dieta Mediterranea (PREDIMED)-Navarra study, a randomised, controlled, parallel trial with three groups of intervention in high cardiovascular risk volunteers, two with a MedDiet and one low-fat control group. Changes in methylation between baseline and 5 years were studied. DNA methylation arrays were analysed by several robust statistical tests and functional classifications. Eight genes related to inflammation and immunocompetence (EEF2, COL18A1, IL4I1, LEPR, PLAGL1, IFRD1, MAPKAPK2, PPARGC1B) were finally selected as changes in their methylation levels correlated with adherence to MedDiet and because they presented sensitivity related to a high variability in methylation changes. Additionally, EEF2 methylation levels positively correlated with concentrations of TNF-alph