ARTÍCULO

Fluorescent nucleoside derivatives as a tool for the detection of concentrative nucleoside transporter activity using confocal microscopy and flow cytometry

Autores: Claudio-Montero, A.; Pinilla-Macua, I.; Fernández-Calotti, P.; Sancho Mateo, Carlos; Lostao Crespo, María del Pilar; Colomer, D.; Grandas, A.; Pastor-Anglada, M.
Título de la revista: MOLECULAR PHARMACEUTICS
ISSN: 1543-8384
Volumen: 12
Número: 6
Páginas: 2158 - 2166
Fecha de publicación: 2015
Resumen:
The abundance and function of transporter proteins at the plasma membrane is likely to be crucial in drug responsiveness. The detection of Human Concentrative Nucleoside Transporters (hCNTs) function is of interest to predict drug sensitivity due to their ability to transport most nucleoside-derived drugs. In the present study two fluorescent nucleoside analogues, Uridine-furan and Etheno-cytidine, were evaluated as tools to study in vivo nucleoside transporter-related functions. These two molecules showed high affinity interactions with hCNT1 and hCNT3 being also hCNT substrates. Both fluorescent microscopy and flux cytometry experiments showed that Uridine-furan uptake was better suited for distinguishing cells that express or not hCNT1 or hCNT3. These data highlight the usefulness of fluorescent nucleoside derivatives as long as they fulfil the requirements of confocal microscopy and flow cytometry for in vivo analysis of hCNT-related function. Fluorescent Nucleoside Derivatives as a Tool for the Detection of Concentrative Nucleoside Transporter Activity Using Confocal Microscopy and Flow Cytometry. Available from: https://www.researchgate.net/publication/275661168_Fluorescent_Nucleoside_Derivatives_as_a_Tool_for_the_Detection_of_Concentrative_Nucleoside_Transporter_Activity_Using_Confocal_Microscopy_and_Flow_Cytometry [accessed Mar 27, 2017].