Nuestros investigadores

Publicaciones científicas más recientes (desde 2010)

Autores: Baraibar Argota, Iosune; Román Moreno, Marta; Rodríguez Remírez, María; et al.
Revista: CANCERS
ISSN 2072-6694  Vol. 12  Nº 11  2020  págs. 31-69
Autores: Fernández Montero, Alejandro (Autor de correspondencia); García Ros, David; Sánchez Tainta, Ana; et al.
ISSN 1076-2752  Vol. 61  Nº 9  2019  págs. 729 - 734
Objective: Our aim was to measure the association of burnout syndrome with insulin resistance in the context of a workplace health intervention. Methods: One-year intervention program (2015 to 2016) within a university workplace. Participants (n=55) were categorized by the presence or absence of burnout syndrome at baseline using the Maslach Burnout Inventory. Insulin resistance was calculated by the triglyceride glucose index (TyG). The Mediterranean Diet adherence score and several fitness tests were completed by the participants. Results: Although participants with prevalent burnout syndrome at baseline improved their physical fitness and diet scores more than participants without burnout syndrome, multiple linear regression analyses showed that participants with prevalent burnout syndrome at baseline had increased TyG index compared with participants without burnout syndrome (beta=0.18; 95% CI, 0.01 to 0.34). Conclusion: Burnout syndrome may be associated with insulin resistance, despite improvements in diet and fitness.
Autores: De Andrea, Carlos Eduardo; Abengozar Muela, Marta; García Ros, David; et al.
ISSN 0023-6837  Vol. 99  Nº Supl. 1  2019  págs. 1820
Autores: Ayestarán Aldaz, A.; García Ros, David; Sánchez Tainta, Ana; et al.
ISSN 1132-6255  Vol. 26  Nº 4  2017  págs. 247 - 256
The aim of this study is to analyse the correlation between the main components of physical activity (strength, flexibility and resistance) and quality of life examined in a work environment. Method: In a randomized, controlled trial throughout one year of follow-up patients were evaluated by their quality of life (SF-36 health survey) and their physical exercise (resistance measured by cardiopulmonary stress test; strength and flexibility based on Eurofit Fitness Test Battery). The intervention group received a personalized exercise plan and the control group attended an informative talk with general guidelines. Results: Both groups improved their physical activity, however, Intervention group had better results 48,7 than Control group 40,87 (p=0,03). Workers with better physical activity proved a greater quality of life (OR:6,69; IC95%:1,48-30,20), being the resistance the major contributing component (OR:8,64; IC95%:1,69-44,04). Conclusion: Workers with a higher level of physical exercise are associated with improved quality of life.
Autores: Villalba, M.; López, L.; Redrado Jordán, Miriam; et al.
ISSN 0213-3911  Vol. 32  Nº 9  2016  págs. 929 - 940
Metastatic spread is responsible for the majority of cancer deaths and identification of metastasis-related therapeutic targets is compulsory. TMPRSS4 is a pro-metastatic druggable transmembrane type II serine protease whose expression has been associated with the development of several cancer types and poor prognosis. To study the role and expression of this protease in cancer, we have developed molecular tools (active recombinant proteins and a polyclonal antibody) that can be used for diagnostic purposes and for testing anti-TMPRSS4 drugs. In addition, we have evaluated TMPRSS4 protein expression in several cancer tissue microarrays (TMAs). Full length and truncated TMPRSS4 recombinant proteins maintained the catalytic activity in two different expression systems (baculovirus and E. coli). Sensitivity of the rabbit polyclonal antisera against TMPRSS4 (ING-pAb) outperformed the antibody most commonly used in clinical settings. Analysis by immunohistochemistry in the different TMAs identified a subset of adenocarcinomas, squamous carcinomas, large cell carcinomas and carcinoids of the lung, which may define aggressive tumors. In conclusion, our biological tools will help the characterization of TMPRSS4 activity and protein expression, as well as the evaluation of anti-TMRSS4 drugs. Future studies should determine the clinical value of assessing TMPRSS4 levels in different types of lung cancer.
Autores: López de Aberasturi Soladrero, Arrate; Redrado Jordán, Miriam; Villalba, M.; et al.
ISSN 0304-3835  Vol. 370  Nº 2  2016  págs. 165 - 176
Metastasis involves a series of changes in cancer cells that promote their escape from the primary tumor and colonization to a new organ. This process is related to the transition from an epithelial to a mesenchymal phenotype (EMT). Recently, some authors have shown that migratory cells with an EMT phenotype share properties of cancer stem cells (CSCs), which allow them to form a new tumor mass. The type II transmembrane serine protease TMPRSS4 is highly expressed in some solid tumors, promotes metastasis and confers EMT features to cancer cells. We hypothesized that TMPRSS4 could also provide CSC properties. Overexpression of TMPRSS4 reduces E-cadherin and induces N-cadherin and vimentin in A549 lung cancer cells, supporting an EMT phenotype. These changes are accompanied by enhanced migration, invasion and tumorigenicity in vivo. TMPRSS4 expression was highly increased in a panel of lung cancer cells cultured as tumorspheres (a typical assay to enrich for CSCs). H358 and H441 cells with knocked-down TMPRSS4 levels were significantly less able to form primary and secondary tumorspheres than control cells. Moreover, they showed a lower proportion of ALDH+ cells (examined by FACS analysis) and lower expression of some CSC markers than controls. A549 cells overexpressing TMPRSS4 conferred the opposite phenotype and were also more sensitive to the CSC-targeted drug salinomycin than control cells, but were more resistant to regular chemotherapeutic drugs (cisplatin, gemcitabine and 5-fluorouracil). Analysis of 70 NSCLC samples from patients revealed a very significant correlation between TMPRSS4 expression and CSC markers ALDH (p¿=¿0.0018) and OCT4 (p¿=¿0.0004), suggesting that TMPRSS4 is associated with a CSC phenotype in patients' tumors. These results show that TMPRSS4, in addition to inducing EMT, can also promote CSC features in lung cancer; therefore, CSC-targeting drugs could be an appropriate treatment for TMPRSS4+ tumors.