Nuestros investigadores

María Pilar Gil Sánchez

Publicaciones científicas más recientes (desde 2010)

Autores: Bernad, Isabel; Gil, María Pilar; et al.
ISSN 1476-4172  Vol. 16  Nº 3  2014  págs. 141-143
Fox Fordyce disease (FFD) has been recently described as an adverse effect of laser hair removal. It is an apocrine gland disorder characterized by pruritus and a folliculocentric papular eruption in apocrine sweat gland areas. Different etiologies have been proposed to be the cause of this entity. It has been suggested that a fisical factor could contribute to FFD phatogenesis. We report a new case of FFD after laser hair removal.
Autores: Gil, María Pilar; Bernad, Isabel; et al.
ISSN 0304-5412  Vol. 11  Nº 48  2014  págs. 2864-9
Las fotodermatosis están causadas por una reacción anómala frente al sol y a las fuentes artificiales, generalmente por la radiación ultravioleta. Se dividen en idiopáticas, fototóxicas y fotoalérgicas y secundarias. Su diagnóstico diferencial es difícil por la similitud clínica y la nomenclatura confusa. Los estudios fotobiológicos (el fototest, la fotoprovocación y la prueba de fotoparche) tienen utilidad clínica y son necesarios para el diagnóstico en varias fotodermatosis.
Autores: Gil, María Pilar; España, Agustín;
ISSN 0304-5412  Vol. 11  Nº 48  2014  págs. 2870-2882
Autores: Idoate, Miguel Ángel; Echeveste, José Ignacio; Gil, María Pilar; et al.
ISSN 1018-9068  Vol. 23  Nº 6  2013  págs. 392 - 397
Background: 2D7 and BB1 are thought to be basophil-specific markers. In this study, we tested both antibodies in different skin and mast cell disorders with the aim of determining whether it was possible to differentiate between benign and aggressive presentations of mastocytosis. Methods: Using the antibodies 2D7, BB 1, and c-Kit, we performed an immunohistochemical study of skin biopsy specimens from patients with cutaneous mastocytosis (15 urticaria pigmentosa and telangiectatic macularis eruptive perstans) and liver or bone marrow biopsy specimens from patients with systemic mastocytosis. A basophil leukemia cell line was used as a reference. Peripheral blood basophils from healthy donors were used as controls. Results: We observed intense expression of 2D7 and BB1 in all skin biopsy specimens from patients with cutaneous mastocytosis. Immunostaining of liver and bone marrow specimens from patients with systemic mastocytosis with 2D7 and BB1 antibodies was negative. Specimens from patients with either type of mastocytosis showed similarly strong expression of c-Kit. The basophil cell line showed a 2D7 and a BB1 profile, with intense expression of c-Kit. Peripheral blood basophils exhibited notable immunostaining for 2D7, BB1, and c-Kit. Conclusions: 2D7 and BB1 are expressed in cutaneous mastocytosis, although this expression is lost when mast cell proliferation is systemic, thus reflecting either a different cellular differentiation stage or the presence of basophils in these skin diseases.
Autores: España, Agustín; Gil, María Pilar; et al.
ISSN 0906-6705  Vol. 22  Nº 2  2013  págs. 125-130
Pemphigus vulgaris (PV) is an autoimmune blistering skin disease characterized by suprabasal acantholysis produced as a consequence of desmoglein (Dsg) and non-Dsg autoantibodies binding to several targeting molecules localized on the membrane of keratinocytes. Nitric oxide (NO) may exert a pathogenic function in several immunological processes. We have previously demonstrated that neural nitric oxide synthase (nNOS) plays part in PV acantholysis. Also, our group has described a relevant role for HER [human epidermal growth factor receptor (EGFR) related] isoforms and several kinases such as Src (Rous sarcoma), mammalian target of rapamycin (mTOR) and focal adhesion kinase (FAK), as well as caspases in PV development. Using a passive transfer mouse model of PV, we aimed to investigate the relationship between the increase in nNOS and EGFR, Src, mTOR and FAK kinase upregulation observed in PV lesions. Our results revealed a new function for nNOS, which contributes to EGFR-mediated PV acantholysis through the upregulation of Src, mTOR and FAK. In addition, we found that nNOS participates actively in PV at least in part by increasing caspase-9 and caspase-3 activities. These findings underline the important issue that in PV acantholysis, caspase activation is a nNOS-linked process downstream of Src, mTOR and FAK kinase upregulation.
Autores: Gil, María Pilar; España, Agustín; et al.
Revista: Experimental Dermatology
ISSN 0906-6705  Vol. 21  Nº 4  2012  págs. 254 - 259
Autores: Ferrer, Marta; Idoate, Miguel Ángel; Gil, María Pilar; et al.
Libro:  Environmental and genetic actors in allergy and clinical immunology. Proceedings of the 27th environmental and genetic factors in allergy and clinical immunology; proceedings of the 27 symposium of the Collegium Internationale Allergologicum (CIA)
2010  págs. 72-71