Nuestros investigadores

Manuel Francisco Rubio Vallejo

Publicaciones científicas más recientes (desde 2010)

Autores: Reina, G; Leiva, José; Rubio, Manuel Francisco; et al.
ISSN 0304-5412  Vol. 12  Nº 51  2018  págs. 2991 - 2999
Introduction.: Tetanus and botulism constitute two life-threating infections caused by spore-forming bacteria, Clostridium tetani and Clostridium botulinum, respectively. Tetanus.: Tetanus is caused by tetanospasmine toxin, which inhibits GABA and glicine neurotransmitters release, causing spastic paralysis followed by respiratory failure and severe impairment of autonomic nervous system. Mortality rate is approximately 8-60%, therefore a prompt clinical diagnosis is essential to transfer the patient to intensive care unit and establish supportive care. In addition, the toxin must be neutralized with specific immunoglobulin, spasms must be controlled with benzodiazepines and cardiovascular instability must be managed with labetalol and magnesium sulphate. Botulism.: Botulism is caused by a thermolabile neurotoxin resulting in flaccid paralysis and respiratory failure, with an observed mortality of 5-10%. There are eight different toxins (A-H) produced by several Clostridium specie, responsible for botulism. Infection can occur following food poisoning with preformed toxin (food-borne botulism), by ingestion of food contaminated with Clostridium botulinum spores (infant botulism), by wound contamination or bacteria inhalation if used as a bioweapon. Early respiratory and airway support must be established together with additional measures (antitoxin and antibiotic).
Autores: Fernández-Alonso, M; Reina, G; Rubio, Manuel Francisco; et al.
ISSN 0304-5412  Vol. 12  Nº 49  2018  págs. 2901 - 2909
Corynebacterium, Listeria and Bacillus are ubiquitous gram-positive aerobic bacilli, which colonize environment, animals and human body. Corynebacterium diphtheriae produces diphtheria either cutaneous or respiratory forms, a disease controlled by vaccination only in some countries and with increased incidence due to immigration and vaccine rejection. Listeria monocytgenes is associated with bacteremia and meningitis in pregnant women, neonates, elderly and immunosuppressed patients, although its incidence at a global level remains unknown. Bacillus anthracis is considered a potential bioterrorism agent because of its lethality. Considering that all cutaneous, gastrointestinal and respiratory symptoms of anthrax could be unspecific, diagnostic procedures that allow their detection with greater safety in case of suspicion have been developed. Toxin production, invasiveness and intracellular survival, resistance in the environment and lytic enzymes production are mechanisms of virulence of these species. Other Corynebacterium or Bacillus species are considered opportunistic pathogens and their clinical relevance has increased along the last decades, with the increase of immunosuppressed patients and the use of medical devices and prostheses susceptible of colonization.
Autores: Leiva, José; Fernández-Alonso, M; Rubio, Manuel Francisco; et al.
ISSN 0304-5412  Vol. 12  Nº 50  2018  págs. 2941 - 2951
Autores: Fernández-Montero, Alejandro; Alonso-Alvarez, A; Rodríguez, Ana Belén; et al.
ISSN 1132-6255  Vol. 25  Nº 2  2016  págs. 58-72
La PT en la consulta de Medicina del Trabajo está justificada al tratarse de una prueba diagnóstica con alta sensibilidad, pero al generar un gran número de falsos positivos, precisa posteriormente de una prueba con una alta especificidad como el QTF-GIT para evitar la quimioprofilaxis innecesaria.
Autores: Martínez, Nicolás; Rubio, Manuel Francisco; Yuste, JR;
Título: Response to letter
ISSN 1195-1982  Vol. 21  Nº 3  2014  págs. 220 - 221
Autores: Martínez, Nicolás; Rubio, Manuel Francisco; et al.
ISSN 1195-1982  Vol. 20  Nº 5  2013  págs. 326 - 328
We describe a Schistosoma haematobium infection with asymptomatic eosinophilia, persistently negative urine microscopy, and late seroconversion (7.5months) in a traveler returning from Mali. After initial negative parasitological tests, travel history led to diagnostic cystoscopy, allowing final diagnosis with urine microscopy after the bladder biopsy. The patient was successfully treated with praziquantel. Difficulties in making the diagnosis of schistosomiasis in asymptomatic returning travelers are discussed; we propose a trial treatment in these cases.
Autores: Rubio, Manuel Francisco; et al.
ISSN 0213-005X  Vol. 31  Nº 6  2013  págs. 412 - 413
Autores: Yuste, JR; Alfonso, Matías; Bustos, César Egberto; et al.
ISSN 0300-8126  Vol. 40  Nº 4  2012  págs. 445 - 449
Candida osteomyelitis is a well recognized but infrequent entity. We describe an interesting case of iliac bone C. albicans osteomyelitis as a result of a surgical trauma of an iliac bone for the auto-grafting of a fracture in the lumbar spine. The peri-operative acquisition of Candida was by the inoculation of a yeast colonizing the skin. Remarkably, several risk factors described for Candida infection and candidemia were absent. The patient also presented with a local fistula. The iliac crest was the only bone affected and local pain was the only symptom present in our case. Diagnosis was made by multiple-specimen biopsy obtained by surgery. Treatment with fluconazole was successful.
Autores: del Pozo, José Luis; Francés, M.L.; et al.
ISSN 0391-3988  Vol. 34  Nº 9  2011  págs. 766-770
Effectiveness of amphotericin B alone or in combination with rifampicin or clarithromycin on the killing of Candida species biofilms was investigated in vitro. Amphotericin B was assayed at 0.005 to 10 mg/ml. Rifampin and clarithromycin were assayed at 10 mg/ml. We studied 7 Candida albicans, 3 Candida parapsilosis, 3 Candida glabrata, 3 Candida krusei and 2 Candida tropicalis strains. Biofilms were developed in 96-well, flat-bottomed microtiter plates for 48 hours. A synergistic effect between amphotericin B and clarithromycin was demonstrated against 66.6% of C. parapsilosis, 66.6% of C. glabrata, and 42.8% of C. albicans biofilms. A synergistic effect between amphotericin B and rifampin was demonstrated against 66.6% of C. parapsilosis, 42.8% of C. albicans, and 33.3% of C. glabrata biofilms. No synergistic effect was observed against C. krusei or C. tropicalis biofilms with any of the combinations. Rifampin or clarithromycin alone did not exert any effect on Candida species biofilms. Rifampin or clarithromycin combinations with amphotericin B might be of interest in the treatment of Candida biofilm-related infections.
Autores: Pérez, Alejandra; Fernández-Alonso, M; et al.
ISSN 0025-7753  Vol. 134  Nº 8  2010  págs. 377 - 378
Autores: Yuste, JR; Pastrana, Juan Carlos; Rubio, Manuel Francisco; et al.
Libro:  La clínica y el laboratorio. 22ª ed
Nº Capítulo 34  2015  págs. 763-872
Autores: Yuste, JR; Leiva, José; Rubio, Manuel Francisco; et al.
Libro:  La clínica y el laboratorio. 21ª ed
Nº Capítulo 34  2010  págs. 665 - 752