Nuestros investigadores

Jacobo Paredes Puente

Departamento
Departamento de Ingeniería Biomédica y Ciencias
Escuela de Ingenieros (TECNUN). Universidad de Navarra
Líneas de investigación
Biodispositivos, Biosensores, Microfluidica, Microfabricación, Monitorización, Microbiología, Ingeniería de Tejidos, Propiedades mecánicas de los tejidos, Cultivo de tejidos, Sistemas de cultivo celular
Índice H
8, (Google Scholar, 16/04/2019)
5, (WoS, 12/01/2018)

Publicaciones científicas más recientes (desde 2010)

Autores: Hajian, R; Balderston, S.; Tran, T.; et al.
Revista: NATURE BIOMEDICAL ENGINEERING
ISSN 2157-846X  Vol. 3  2019  págs. 427 - 437
Most methods for the detection of nucleic acids require many reagents and expensive and bulky instrumentation. Here, we report the development and testing of a graphene-based field-effect transistor that uses clustered regularly interspaced short palindromic repeats (CRISPR) technology to enable the digital detection of a target sequence within intact genomic material. Termed CRISPR-Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated CRISPR-associated protein 9 (Cas9) complexed with a specific single-guide RNA and immobilized on the transistor to yield a label-free nucleic-acid-testing device whose output signal can be measured with a simple handheld reader. We used CRISPR-Chip to analyse DNA samples collected from HEK293T cell lines expressing blue fluorescent protein, and clinical samples of DNA with two distinct mutations at exons commonly deleted in individuals with Duchenne muscular dystrophy. In the presence of genomic DNA containing the target gene, CRISPR-Chip generates, within 15min, with a sensitivity of 1.7fM and without the need for amplification, a significant enhancement in output signal relative to samples lacking the target sequence. CRISPR-Chip expands the applications of CRISPR-Cas9 technology to the on-chip electrical detection of nucleic acids.
Autores: Gardeazabal, L.; Yndriago, L.; Crespo-Barreda, A.; et al.
Revista: JOURNAL OF INVESTIGATIVE DERMATOLOGY
ISSN 0022-202X  Vol. 139  Nº 9  2019  págs. S327 - S327
Autores: Tubia, Imanol, (Autor de correspondencia); Prasad, K.; Pérez, Eva ; et al.
Revista: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN 0168-1605  Vol. 283  2018  págs. 65 - 76
Spoilage yeasts detection is the key to improve the quality of alcoholic fermentation beverages such as wine and cider. The metabolic activity of the spoilage yeast causes irreparable damage to many liters of final products every year. Therefore, winemakers and cider-house companies suffer a substantial economic impact. Thus, over the years, many detection techniques have been proposed to control the occurrence of spoilage yeast. Out of the many spoilage yeast genera, Brettanomyces is one of the most commonly encountered in the beverage industry. Leveraging its ability to thrive in wine and cider conditions (low pH, high levels of ethanol, and low oxygenation levels), Brettanomyces can proliferate inside beverage production tanks. Moreover, their resultant by products reduce the quality of the beverage. While the beverage industry has made great strides in detecting harmful organisms, gaps remain. Traditional methods such as microscopy, cell plating, gas chromatography-mass spectrometry, etc. are often imprecise, expensive, and/or complicated. New emerging spoilage yeast detection platforms, such as biosensors and microfluidic devices, aim to alleviate these constraints. Novel platforms have already demonstrated great promise to be a real alternative for in situ and fast detection in the beverage industry. Finally, the review discusses the potential of emerging spoilage yeast detection and treatment methods.
Autores: Tubia, Imanol, (Autor de correspondencia); Paredes, J; Pérez, Eva ; et al.
Revista: SENSORS AND ACTUATORS A-PHYSICAL
ISSN 0924-4247  Vol. 269  2018  págs. 175 - 181
Brettanomyces bruxellensis is considered one of the most relevant spoilage yeasts in the production of alcoholic beverages, especially for wine and cider. During fermentation and later storage, these yeasts can cause changes in the characteristics of the product, ruining the aroma and taste. The presence of Brettanomyces causes a decrease in the quality of the final products and important economic losses. The current work presents a detection method based on impedance spectroscopy analysis using label-free interdigitated microelectrode (IDE) based sensors for spoilage yeast detection. Different conditions (static and stirring) were tested in Brettanomyces cultures inside reactors in order to evaluate the growth behavior. Our results indicate a faster response and an 8% increase of the relative variation of the impedance under stirring condition due to biofilm formation onto the surface of the sensors. Equivalent circuit analysis also confirmed that the difference was caused by the larger biofilm formation under dynamic conditions. The results suggest that this technology could be applied for the early detection of spoilage yeast in wine and cider industries, providing more efficient methods to achieve a higher quality of the final products. (C) 2017 Elsevier B.V. All rights reserved.
Autores: Tubia, Imanol; Paredes, J; Pérez, Eva ; et al.
Revista: BIOSENSORS AND BIOELECTRONICS
ISSN 0956-5663  Vol. 102  2018  págs. 432 - 438
Brettanomyces is a yeast species responsible for wine and cider spoilage, producing volatile phenols that result in off-odors and loss of fruity sensorial qualities. Current commercial detection methods for these spoilage species are liable to frequent false positives, long culture times and fungal contamination. In this work, an interdigitated (IDE) biosensor was created to detect Brettanomyces using immunological reactions and impedance spectroscopy analysis. To promote efficient antibody immobilization on the electrodes¿ surface and to decrease non-specific adsorption, a Self-Assembled Monolayer (SAM) was developed. An impedance spectroscopy analysis, over four yeast strains, confirmed our device's increased efficacy. Compared to label-free sensors, antibody biosensors showed a higher relative impedance. The results also suggested that these biosensors could be a promising method to monitor some spoilage yeasts, offering an efficient alternative to the laborious and expensive traditional methods.
Autores: Tubia, Imanol, (Autor de correspondencia); Apezteguia, A.; et al.
Revista: SENSORS AND ACTUATORS A-PHYSICAL
ISSN 0924-4247  Vol. 277  2018  págs. 1 - 7
Impedance spectroscopy analysis (IS) has awakened a great interest for many industrial applications and sectors for the implementation of novel monitoring capabilities. More specifically, microelectrode-based sensors are increasingly being used to analyze electrical or electrochemical changes in liquid samples, as well as other effects such as biofouling, particle adhesion, etc. However, real environmental conditions are usually subjected physiochemical changes that affect the impedance measurement. In this context, it is difficult to isolate the effect of only one parameter (Le., conductivity of the medium) from the other ones. This work is focused specifically on the analysis of the influence of temperature and pH on the impedance measurements. Different experiments were carried out using interdigitated microelectrodes (IDE) sensors for a geometry range in wine samples to adjust a proposed mathematical model of the impedance behavior. In the case of fermentation processes of alcoholic beverages, this methodology will help to isolate the chemical changes measured by impedance from temperature or pH variation. This model also provides the significance of the effect of each parameter on the impedance values. After the experimental validation, the model was used to correct the impedance values accordingly to the variation of each parameter showing its applicability to the real field. Finally, the proposed methodology can be easily applied and extended to other environments and sensors types. (C) 2018 Elsevier B.V. All rights reserved.
Autores: Sadlowski, C.; Balderston, S.; Sandhu, M. ; et al.
Revista: LAB ON A CHIP
ISSN 1473-0197  Vol. 18  Nº 21  2018  págs. 3230 - 3238
Studies of heterochronic parabiosis, where two animals of different ages are joined surgically, provided proof-of-principle results that systemic proteins have broad age-specific effects on tissue health and repair. In an effort to identify these systemic proteins, we previously developed a method to selectively label the proteome of only one animal joined in parabiosis utilizing bio-orthogonal non-canonical amino acid tagging (BONCAT), which can metabolically label proteins during their de novo synthesis by incorporating a methionine substitute, azido-nor-leucine (ANL), in cells expressing a mutant methionyl-tRNA synthetase (MetRS(L274G)). Once labeled, we can selectively identify the proteins produced by the MetRS(L274G) transgenic mouse in the setting of heterochronic parabiosis. This approach enabled the detection of several rejuvenating protein candidates from the young parabiont, which were transferred to the old mammalian tissue through their shared circulation. Although BONCAT is a very powerful technology, the challenges associated with its complexity including large starting material requirements and cost of ANL-labeled protein detection, such as modified antibody arrays and mass spectrometry, limit its application. Herein, we propose a lab-on-a-chip technology, termed Click-A+Chip for facile and rapid digital detection of ANL-labeled proteomes present in minute amount of sample, to replace conventional assays. Click-A+Chip is a graphene-based field effect biosensor (gFEB) which utilizes novel on-chip click-chemistry to specifically bind to ANL-labeled biomolecules. In this study, Click-A+Chip is utilized for the capture of ANL-labeled proteins transferred from young to old parabiotic mouse partners. Moreover, we were able to identify the young-derived ANL-labeled Lif-1 and leptin in parabiotic systemic milieu, confirming previous data as well as providing novel findings on the relative levels of these factors in young versus old parabionts. Summarily, our results demonstrate that Click-A+Chip can be used for rapid detection and identification of ANL-labeled proteins, significantly reducing the sample size, complexity, cost and time associated with BONCAT analysis.
Autores: Aran, K.; Chooljian, M.; Paredes, J; et al.
Revista: SCIENCE TRANSLATIONAL MEDICINE
ISSN 1946-6234  Vol. 9  Nº 380  2017 
Noninvasive immunization technologies have the potential to revolutionize global health by providing easy-to-administer vaccines at low cost, enabling mass immunizations during pandemics. Existing technologies such as transdermal microneedles are costly, deliver drugs slowly, and cannot generate mucosal immunity, which is important for optimal immunity against pathogens. We present a needle-free microjet immunization device termedMucoJet, which is a three-dimensional microelectromechanical systems-based drug delivery technology. MucoJet is administered orally, placed adjacent to the buccal tissue within the oral cavity, and uses a self-contained gas-generating chemical reaction within its twocompartment plastic housing to produce a high-pressure liquid jet of vaccine. We show that the vaccine jet ejected from the MucoJet device is capable of penetrating the buccal mucosal layer in silico, in porcine buccal tissue ex vivo, and in rabbits in vivo. Rabbits treated with ovalbumin by MucoJet delivery have antibody titers of anti-ovalbumin immunoglobulins G and A in blood serum and buccal tissue, respectively, that are three orders of magnitude higher than rabbits receiving free ovalbumin delivered topically by a dropper in the buccal region. MucoJet has the potential to accelerate the development of noninvasive oral vaccines, given its ability to elicit antibody production that is detectable locally in the buccal tissue and systemically via the circulation.
Autores: Becerro, S.; Paredes, J; Mujika, Maite; et al.
Revista: IEEE SENSORS JOURNAL
ISSN 1530-437X  Vol. 16  Nº 7  2016  págs. 1856 - 1864
Bacterial biofilms led to numerous problems in a wide variety of sectors as the medical environment, the food and water industry, or the naval sector. Completely developed biofilms are nearly impossible to eliminate due to the high antibiotic resistance these complex systems present. The lack of evidential indicators of their presence at the first stages of development makes antimicrobial treatments late and inadequate. Therefore, it is necessary to find new methods for the early detection of biofilm development in order to improve the efficiency of treatments by exposing bacterial cells before encapsulation in the extracellular matrix. For this purpose, this paper presents a real-time analysis of bacterial adhesion and biofilm growth by means of electrochemical measurements. Cyclic voltammetry and differential pulse voltammetry were performed with thin-film interdigitated microelectrode-based sensors. More sensitive and selective measurements were obtained with the second technique. Bacterial adhesion was detected 1 h after the initial inoculum, and three different redox centers were identified on bacterial surfaces. Finally, bacterial biofilm growth phases (lag, exponential, and stationary) were identified through the electrochemical measurements.
Autores: Viswanathan, S.; Narayanan, T.; Aran, K.; et al.
Revista: MATERIALS TODAY
ISSN 1369-7021  Vol. 18  Nº 9  2015  págs. 513 - 522
Chronic diseases are becoming more prevalent, and the complexities of managing patients continue to escalate, since their care must be balanced between the home and clinical settings. Diabetes is the most advanced example, where self-monitoring has been shown to be necessary. Glucometers are point-of-care (POC) devices that have become standard platforms at home and clinical settings. Similarly, many other POC biosensors have also been developed. Enzymes are often used in these sensors because of their specificity and the reaction products can be electrochemically transduced for the measurement. When enzymes are immobilized to an electronically active substrate, enzymatic reactions can be transduced by direct electron transport. This paper describes an approach for the development of graphene-based POC devices. This includes modifying enzymes for improved performance, developing methods to bind them to the graphene surface, incorporation of the functionalized graphene on a field-effect transistor (FET), and integration into a microfluidic device suitable for home use. This paper describes an approach for the development of a graphene-based POC biosensor platform using glucose as an example of target molecule.
Autores: Paredes, J; Fink, K.; Novak, R.; et al.
Revista: SENSORS AND ACTUATORS B-CHEMICAL
ISSN 0925-4005  Vol. 216  2015  págs. 263 - 270
Microfluidic devices play an increasingly important role in healthcare-related fields, but integration of electrodes and electronic components has been restricted at the prototyping stage of product development by a limited range of fabrication methods. In this work a new fabrication methodology is presented for embedding metallic microelectrodes in thermoplastic microfluidic devices. Microelectrodes are fabricated on steel wafers by means of photolithographic patterning and electrodeposition and then transferred to a thermoplastic sheet using hot embossing, resulting in embedded metal electrodes flush with the polymer surface. The unique shape of the microelectrodes provides an anchoring mechanism that ensures structural stability and reliability of the devices. A wide variety of thermoplastics can be used in this process including polycarbonate, polymethylmethacrylate (PMMA), and cyclic olefin copolymer (COC). Devices are assembled by a solvent-assisted bonding process, after drilling the inlets and outlets. This method allows for rapid fabrication of robust embedded electrodes and wiring connections from a broad range of metals for thermoplastic microfluidic devices. Finally, embedded interdigitated microelectrodes are used to measure conductivity within a microchannel via impedance spectroscopy analysis. The use of this technology is relevant to a wide range of analytical applications. (C) 2015 Elsevier B.V. All rights reserved.
Autores: Aran,K.; Paredes, J; Rafi, M.; et al.
Revista: ADVANCED MATERIALS
ISSN 0935-9648  Vol. 27  Nº 8  2015  págs. 1433 - 1436
A digital point-of-care biosensor for measuring reactive oxygen species is presented based on novel reactive oxygen species responsive polymer-based electrodes. The biosensor is able to detect a drug-induced liver injury by monitoring the oxidative stress in the blood.
Autores: Paredes, J; Becerro, S.; Arana, Sergio;
Revista: SENSORS AND ACTUATORS B-CHEMICAL
ISSN 0925-4005  Vol. 195  2014  págs. 667 - 676
Bacterial biofilms are presented in many different environments causing a wide variety of infectious processes. Biofilms at their mature stage are difficult to eradicate because of their inherent resistance to antimicrobial agents. Easy-to-integrate and in situ detection tools would provide early detection of bacterial presence allowing efficient prophylactic actions. Impedance microbiology has been postulated as a suitable technique that allows monitoring of bacterial biofilm growths in real time. In this work four different culturing setups were developed as testing platforms for measuring real time microbiological cultures that could mimic real field environments. Results suggest that the position of the sensors in regard to the dynamic conditions of the culture might affect the sensitivity and the target parameter. Capacitance and resistance are associated to different biological effects, surface coating and conductivity changes respectively. Relative variations of electrical parameters were recorded in the lab obtaining significant changes in few hours post-infection. It has been proven that biological coating cause largest variations in capacitance, up to 60%, while metabolic activity affects more the resistance giving a variation up to 15%. Fitting analysis has confirmed experimental results showing also the effect of the dead/alive ratio. (C) 2014 Elsevier B.V. All rights reserved.
Autores: Paredes, J; Becerro, S.; Arana, Sergio;
Revista: JOURNAL OF MICROBIOLOGICAL METHODS
ISSN 0167-7012  Vol. 100  2014  págs. 77 - 83
Impedance microbiology (IM) is a known technique that has been applied during the last decades to detect the presence of microorganisms in real samples in different fields: food industry, healthcare, environment, etc. Bacterial biofilms however have not been so far studied despite the fact that they are the most common microbiological formation and that they present resistance to antimicrobial agents. In situ early detection of bacterial biofilm is still a challenge nowadays that causes huge impact in many different scenarios. The ability to detect biofilm generation early will allow better and more efficient treatments preventing high costs and important problems. In this work a new performance of this technique with interdigitated microelectrode sensors (IDE) is proposed. A specific culturing setup where the sensors have been integrated in Petri Dishes has been developed. From the results it can be highlighted that low frequencies are more sensitive for detection than higher ones. The results achieved record variations of approximately 40% in the equivalent serial resistance after 10 h of culture. Electrical models have been successfully simulated to find the electrical behavior of the development of biofilms. Variations in both the capacitance and resistance were recorded during the growth of the microbes. (C) 2014 Elsevier B.V. All rights reserved.
Autores: Paredes, J; Alonso-Arce, Maykel; Schmidt, C.; et al.
Revista: BIOMEDICAL MICRODEVICES
ISSN 1387-2176  Vol. 16  Nº 3  2014  págs. 365 - 374
Central venous catheters (CVC) are commonly used in clinical practice to improve a patient's quality of life. Unfortunately, there is an intrinsic risk of acquiring an infection related to microbial biofilm formation inside the catheter lumen. It has been estimated that 80 % of all human bacterial infections are biofilm-associated. Additionally, 50 % of all nosocomial infections are associated with indwelling devices. Bloodstream infections account for 30-40 % of all cases of severe sepsis and septic shock, and are major causes of morbidity and mortality. Diagnosis of bloodstream infections must be performed promptly so that adequate antimicrobial therapy can be started and patient outcome improved. An ideal diagnostic technology would identify the infecting organism(s) in a timely manner, so that appropriate pathogen-driven therapy could begin promptly. Unfortunately, despite the essential information it provides, blood culture, the gold standard, largely fails in this purpose because time is lost waiting for bacterial or fungal growth. This work presents a new design of a venous access port that allows the monitoring of the inner reservoir surface by means of an impedimetric biosensor. An ad-hoc electronic system was designed to manage the sensor and to allow communication with the external receiver. Historic data recorded and stored in the device was used as the reference value for the detection of bacterial biofilm. The RF communication system sends an alarm signal to the external receiver when a microbial colonization of the port occurs. The successful in vitro analysis of the biosensor, the electronics and the antenna of the new indwelling device prototype are shown. The experimental conditions were selected in each case as the closest to the clinical working conditions for the smart central venous catheter (SCVC) testing. The results of this work allow a new generation of this kind of device that could potentially provide more efficient treatments for catheter-related infections.
Autores: Paredes, J; Becerro, S.; Arizti, Fernando José; et al.
Revista: SENSORS AND ACTUATORS B-CHEMICAL
ISSN 0925-4005  Vol. 178  2013  págs. 663 - 670
Bacterial biofilms are a common cause of persistent and chronic infections, mostly related to implantable devices. In this context, Staphylococcus species are the most relevant microorganisms involved in causing high costs for the healthcare system. New diagnostic and/or therapeutic tools should be developed by providing in vivo analysis of the specific physiological parameters directly related to the status of the indwelling device. Monitoring the biofilm's biological evolution will allow an earlier diagnostic. Impedance microbiology is suggested as a proper technique for directly measuring the development of bacterial biofilms generated inside intravascular catheters. In this study we propose interdigitated microelectrode biosensors be integrated within those implantable devices. In vitro assays have been carried out in order to characterize this methodology as a detection and monitoring tool for bacterial biofilm development. Impedance spectroscopy (IS) was implemented in 96-well microtiter plates, leading to positive results in detecting and monitoring bacterial biofilm development. Two Staphylococcus aureus and two Staphylococcus epidermidis strains were successfully monitored during a 20-h culture, and results show that the low range of the frequency is the most suitable setting for measuring the maximum relative changes. (c) 2013 Elsevier B.V. All rights reserved.
Autores: Paredes, J; Becerro, S.; Arizti, Fernando José; et al.
Revista: BIOSENSORS AND BIOELECTRONICS
ISSN 0956-5663  Vol. 38  Nº 1  2012  págs. 226 - 232
Detection of device-associated infectious processes is still an important clinical challenge. Bacteria grow adhered to the device surfaces creating biofilms that are resistant to antimicrobial agents, increasing mortality and morbidity. Thus there is need of a surgical procedure to remove the indwelling infected device. The elevated cost of these procedures, besides patients discomfort and increased risks, highlights the need to develop more efficient, accurate and rapid detection methods. Biosensors integrated with implantable devices will provide an effective diagnostic tool. In vivo, rapid and sensitive detection of bacteria attached to the device surfaces will allow efficient treatments. Impedance spectroscopy technique would be an adequate tool to detect the adherence and the growth of the microorganism by monitoring the impedance characteristics. In this work a label-free interdigitated microelectrode (IDAM) biosensor has been developed to be integrated with implantable devices. Impedance characterization of Staphylococcus epidermidis biofilms has been performed achieving electrical monitoring of the bacterial growths in a few hours from the onset of the infection. This pathogen represents the most common microorganism related to intravascular catheters associated infections. The experimental setup presented in this work, a modified CDC biofilm reactor, simulates the natural environment conditions for bacterial biofilm development. The results prove that the low range of frequency is the most suitable setting for monitoring biofilm development. Our findings prove the effectiveness of this technique which shows variations of 59% in the equivalent serial capacitance component of the impedance. (C) 2012 Elsevier B.V. All rights reserved.
Autores: Paredes, J; Tubia, Imanol; Arana, Sergio;
Libro:  Handbook of online and near-real-time methods in microorganism detection
2017  págs. 1 - 1
Autores: Paredes, J; Chooljian, M.; Liepmann, D.;
Libro:  Design for advanced manufacturing: technologies and processes
2017  págs. 379 - 388
Detailed coverage of the latest advances in manufacturing and product design. Edited by a past president of the Society of Manufacturing Engineers, this comprehensive resource features contributions from a host of international manufacturing and design experts. You will get thorough explanations of the entire range of advanced manufacturing processes and technologies as well as practical instructions and technical guidance. Design for Advanced Manufacturing: Technologies and Processes covers: ¿ Manufacturing with lasers ¿ Conventional laser processes ¿ Cleaning parts via lasers ¿ Forming parts via lasers ¿ Texturing parts via lasers ¿ Additive processes (3D printing) ¿ Conventional and micro-level additive manufacturing ¿ Micro parts and micro fabrication ¿ Advanced technologies for machining and welding at microscopic levels ¿ Design issues ¿ Production rates ¿ Process comparisons ¿ And much more The book also addresses unique processes such as: ¿ MICA Freeform ¿ LIGA ¿ Electron beam polishing ¿ Magnetic abrasive finishing ¿ Ion beam machining ¿ Micro stereolithography ¿ Micro origami for assembly ¿ Micro machining, welding, finishing ¿ Electrospinning If you've been looking for a comprehensive, quick, and convenient source of answers to all your advanced processes questions, your search ends here.
Autores: Becerro, S.; Paredes, J; Arana, Sergio;
Libro:  XIII Mediterranean Conference on Medical and Biological Engineering and Computing 2013
2014  págs. 880 - 883
Adhesion and growth of bacterial biofilms causes numerous problems in a wide variety of sectors, and more particularly, in those related to the medical environment or the industry. Treatment and disposal of this kind of infections is often hampered by the antibiotic resistance of biofilms as well as by a total lack of symptoms in the early stages. Therefore, it is necessary to find new methods for the early detection of biofilm development so as to improve the efficiency of treatments and to reduce the health complications suffered by patients. For this purpose, this paper focuses on the design and development of interdigitated microelectrode based biosensors that allow the detection of bacterial adhesion since the first steps of biofilm generation through impedance spectroscopy and electrochemistry. Both techniques have been proved as suitable tools for biofilm sensing and the results of the monitorization of bacterial biofilms of S. epidermidis in culture medium are presented. While variations of 40 % within a few hours of incubation have been achieved with impedimetric monitoring, electrochemistry increases both selectivity and sensitivity of the recorded measurements (variations of 60% have been obtained). Moreover, a multiparametric design for electrochemical and temperature measurements that will allow to obtain additional information of bacterial activity is presented.
Autores: Paredes, J;
2015 
Las biocapas bacterianas son estructuras tridimensionales formadas por bacterias de una o varias especies y una matriz extracelular que dota a estos sistemas de características únicas. La más importante es la resistencia a los agentes antimicrobianos que hace de eliminación un verdadero problema en todo tipo de entornos, industrial, alimentario, sanitario. La detección precoz de estos sistemas microbiológicos es una de las claves en la eficacia de los tratamientos de estos micro-bio-sistemas. Las biocapas bacterianas son en muchos casos el origen de infecciones asociadas a los catéteres intravasculares, que resultan difícilmente tratables dando lugar a patologías crónicas en pacientes afectados por otras enfermedades. El objetivo de este trabajo es, por tanto, el desarrollo de un biosensor de medida de impedancia capaz de ser integrado en estos dispositivos para mejorar el diagnóstico de los procesos infecciosos.

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Jacobo finalizo los estudios de Ingenieri­a Industrial por la Universidad de Navarra en diciembre de 2007. Durante este periodo realizo colaboraciones de investigacion en los departamentos de materiales y de ingenieri­a mecanica, en donde mas adelante completo su proyecto fin de carrera. En enero de 2008 curso un Master en Ingenieri­a Biomedica formando parte de la primera promocion de dichos estudios de postgrado y para cual consiguio una beca del grupo Santander. Realizo el proyecto fin de master en el Centro de Estudios e Investigaciones Tecnicas (CEIT) que resultari­a en el comienzo del proyecto de Tesis. En 2009 se incorpora al CEIT en el area de Microsistemas y Microelectronica en donde realizo la tesis doctoral con una beca de la Asociacion de Amigos de la Universidad de Navarra, que finalizo en octubre de 2012 - Desarrollo de un sistema para el analisis impedimetrico de biocapas bacterianas incluyendo biosensores, protocolos y plataforma experimental y su aplicacion en implantes de acceso venoso central. Se trataba de una tesis aplicada al diagnostico precoz de procesos infecciosos en dispositivos implantados que obtuvo una calificacion de sobresaliente cum laude por unanimidad. Fruto del desarrollo de la tesis se han publicado hasta cinco arti­culos en revistas indexadas, asi­ como diversas comunicaciones en congresos internacionales. Durante este periodo pudo colaborar en otros proyectos de investigacion de la li­nea de bioMEMS. En este marco superviso el trabajo de alumnos colaboradores y codirigio tres proyectos fin de carrera en el ambito de instrumentacion. Ademas, colaboro como ayudante de la asignatura de Tecnicas biologicas en el grado de Ingenieri­a Biomedica en Tecnun, impartiendo clases practicas y teoricas. Durante el primer semestre del curso 2012-2013 fue nombrado profesor ayudante de la asignatura de Introduccion a la biomedicina en el mismo grado. Tras la finalizacion de la tesis, completo una estancia postdoctoral de 22 meses en la Universidad de California, Berkeley en el Departamento de Bioingenieri­a bajo la supervision del Prof. Dorian Liepmann. Durante este periodo ha participado en diferentes proyectos de investigacion en el ambito de aplicaciones biomedicas. Ha colaborado supervisado tres alumnos colaboradores y dos alumnos de doctorado, y ha tenido la oportunidad de impartir algo de docencia en la asignatura de BioMEMS and Bionanotechnology laboratory del grado de bioingenieri­a. Actualmente esta vinculado al departamento de Ingenieri­a Biomedica de Tecnun, en donde desarrolla su labor académica: Imparte docencia en los programas grado y master en ingenieri­a biomedica. Asignaturas como Biomateriales polimericos, biomateriales y biocompatibilidad, Ingenieri­a de Tejidos y Medicina Regenerativa. Por otro lado desarrolla su investigacion dentro del nuevo grupo de Ingenieria de Tejidos en el Departamento de Ingenieri­a Biomedica y Ciencias. Esta involucrado en diferentes proyectos de investigacion, supervisa el trabajo de dos alumnos de doctorado como codirector, asi­ como de varios alumnos colaboradores del grupo. Es coautor de diversas publicaciones científicas, patentes, contribuciones a congresos y capitulos de libros.