Revistas
Autores:
Paredes, J; Cortizo-Lacalle, D.; Imaz, A.; et al.
Revista:
SCIENTIFIC REPORTS
ISSN:
2045-2322
Año:
2022
Vol.:
12
N°:
1
Págs.:
3898
Mechanical characterization supposes a key step in the development of cultured meat to help mimicking the sensorial properties of already existing commercial products based on traditional meat. This work presents two well stablished methods that can help studying cultured meat mechanical characteristics: texture profile analysis (double compression test) and rheology. These techniques provide data about the elastic and viscous behaviour of the samples but also values about other texture characteristics such as springiness, cohesiveness, chewiness and resilience. In this work, we present a comparison of cultured meat-based samples with commercial of the shelf common meat products (sausage, turkey and chicken breast). Results show that both Young¿s and Shear modulus in the cultured meat samples can be compared to commercial products in order to understand its properties. The texture characteristics for the cultured meat studied, show values within the range of commercial products. These results demonstrate the applicability of this methodology for the adjustment of mechanical properties of cultured meat products
Revista:
POLYMERS
ISSN:
2073-4360
Año:
2022
Vol.:
14
N°:
7
Págs.:
1311
The electrospinning of hybrid polymers is a versatile fabrication technique which takes advantage of the biological properties of natural polymers and the mechanical properties of synthetic polymers. However, the literature is scarce when it comes to comparisons of blends regarding coatings and the improvements offered thereby in terms of cellular performance. To address this, in the present study, nanofibrous electrospun scaffolds of polycaprolactone (PCL), their coating and their blend with gelatin were compared. The morphology of nanofibrous scaffolds was analyzed under field emission scanning electron microscopy (FE-SEM), indicating the influence of the presence of gelatin. The scaffolds were mechanically tested with tensile tests; PCL and PCL gelatin coated scaffolds showed higher elastic moduli than PCL/gelatin meshes. Viability of mouse embryonic fibroblasts (MEF) was evaluated by MTT assay, and cell proliferation on the scaffold was confirmed by fluorescence staining. The positive results of the MTT assay and cell growth indicated that the scaffolds of PCL/gelatin excelled in comparison to other scaffolds, and may serve as good candidates for tissue engineering applications.
Autores:
Hajian, R; Balderston, S.; Tran, T.; et al.
Revista:
NATURE BIOMEDICAL ENGINEERING
ISSN:
2157-846X
Año:
2019
Vol.:
3
Págs.:
427 - 437
Most methods for the detection of nucleic acids require many reagents and expensive and bulky instrumentation. Here, we report the development and testing of a graphene-based field-effect transistor that uses clustered regularly interspaced short palindromic repeats (CRISPR) technology to enable the digital detection of a target sequence within intact genomic material. Termed CRISPR-Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated CRISPR-associated protein 9 (Cas9) complexed with a specific single-guide RNA and immobilized on the transistor to yield a label-free nucleic-acid-testing device whose output signal can be measured with a simple handheld reader. We used CRISPR-Chip to analyse DNA samples collected from HEK293T cell lines expressing blue fluorescent protein, and clinical samples of DNA with two distinct mutations at exons commonly deleted in individuals with Duchenne muscular dystrophy. In the presence of genomic DNA containing the target gene, CRISPR-Chip generates, within 15min, with a sensitivity of 1.7fM and without the need for amplification, a significant enhancement in output signal relative to samples lacking the target sequence. CRISPR-Chip expands the applications of CRISPR-Cas9 technology to the on-chip electrical detection of nucleic acids.
Revista:
SENSORS AND ACTUATORS A-PHYSICAL
ISSN:
0924-4247
Año:
2018
Vol.:
277
Págs.:
1 - 7
Impedance spectroscopy analysis (IS) has awakened a great interest for many industrial applications and sectors for the implementation of novel monitoring capabilities. More specifically, microelectrode-based sensors are increasingly being used to analyze electrical or electrochemical changes in liquid samples, as well as other effects such as biofouling, particle adhesion, etc. However, real environmental conditions are usually subjected physiochemical changes that affect the impedance measurement. In this context, it is difficult to isolate the effect of only one parameter (Le., conductivity of the medium) from the other ones. This work is focused specifically on the analysis of the influence of temperature and pH on the impedance measurements. Different experiments were carried out using interdigitated microelectrodes (IDE) sensors for a geometry range in wine samples to adjust a proposed mathematical model of the impedance behavior. In the case of fermentation processes of alcoholic beverages, this methodology will help to isolate the chemical changes measured by impedance from temperature or pH variation. This model also provides the significance of the effect of each parameter on the impedance values. After the experimental validation, the model was used to correct the impedance values accordingly to the variation of each parameter showing its applicability to the real field. Finally, the proposed methodology can be easily applied and extended to other environments and sensors types. (C) 2018 Elsevier B.V. All rights reserved.
Revista:
BIOSENSORS AND BIOELECTRONICS
ISSN:
0956-5663
Año:
2018
Vol.:
102
Págs.:
432 - 438
Brettanomyces is a yeast species responsible for wine and cider spoilage, producing volatile phenols that result in off-odors and loss of fruity sensorial qualities. Current commercial detection methods for these spoilage species are liable to frequent false positives, long culture times and fungal contamination. In this work, an interdigitated (IDE) biosensor was created to detect Brettanomyces using immunological reactions and impedance spectroscopy analysis. To promote efficient antibody immobilization on the electrodes¿ surface and to decrease non-specific adsorption, a Self-Assembled Monolayer (SAM) was developed. An impedance spectroscopy analysis, over four yeast strains, confirmed our device's increased efficacy. Compared to label-free sensors, antibody biosensors showed a higher relative impedance. The results also suggested that these biosensors could be a promising method to monitor some spoilage yeasts, offering an efficient alternative to the laborious and expensive traditional methods.
Revista:
SENSORS AND ACTUATORS A-PHYSICAL
ISSN:
0924-4247
Año:
2018
Vol.:
269
Págs.:
175 - 181
Brettanomyces bruxellensis is considered one of the most relevant spoilage yeasts in the production of alcoholic beverages, especially for wine and cider. During fermentation and later storage, these yeasts can cause changes in the characteristics of the product, ruining the aroma and taste. The presence of Brettanomyces causes a decrease in the quality of the final products and important economic losses. The current work presents a detection method based on impedance spectroscopy analysis using label-free interdigitated microelectrode (IDE) based sensors for spoilage yeast detection. Different conditions (static and stirring) were tested in Brettanomyces cultures inside reactors in order to evaluate the growth behavior. Our results indicate a faster response and an 8% increase of the relative variation of the impedance under stirring condition due to biofilm formation onto the surface of the sensors. Equivalent circuit analysis also confirmed that the difference was caused by the larger biofilm formation under dynamic conditions. The results suggest that this technology could be applied for the early detection of spoilage yeast in wine and cider industries, providing more efficient methods to achieve a higher quality of the final products. (C) 2017 Elsevier B.V. All rights reserved.
Autores:
Sadlowski, C.; Balderston, S.; Sandhu, M. ; et al.
Revista:
LAB ON A CHIP
ISSN:
1473-0197
Año:
2018
Vol.:
18
N°:
21
Págs.:
3230 - 3238
Studies of heterochronic parabiosis, where two animals of different ages are joined surgically, provided proof-of-principle results that systemic proteins have broad age-specific effects on tissue health and repair. In an effort to identify these systemic proteins, we previously developed a method to selectively label the proteome of only one animal joined in parabiosis utilizing bio-orthogonal non-canonical amino acid tagging (BONCAT), which can metabolically label proteins during their de novo synthesis by incorporating a methionine substitute, azido-nor-leucine (ANL), in cells expressing a mutant methionyl-tRNA synthetase (MetRS(L274G)). Once labeled, we can selectively identify the proteins produced by the MetRS(L274G) transgenic mouse in the setting of heterochronic parabiosis. This approach enabled the detection of several rejuvenating protein candidates from the young parabiont, which were transferred to the old mammalian tissue through their shared circulation. Although BONCAT is a very powerful technology, the challenges associated with its complexity including large starting material requirements and cost of ANL-labeled protein detection, such as modified antibody arrays and mass spectrometry, limit its application. Herein, we propose a lab-on-a-chip technology, termed Click-A+Chip for facile and rapid digital detection of ANL-labeled proteomes present in minute amount of sample, to replace conventional assays. Click-A+Chip is a graphene-based field effect biosensor (gFEB) which utilizes novel on-chip click-chemistry to specifically bind to ANL-labeled biomolecules. In this study, Click-A+Chip is utilized for the capture of ANL-labeled proteins transferred from young to old parabiotic mouse partners. Moreover, we were able to identify the young-derived ANL-labeled Lif-1 and leptin in parabiotic systemic milieu, confirming previous data as well as providing novel findings on the relative levels of these factors in young versus old parabionts. Summarily, our results demonstrate that Click-A+Chip can be used for rapid detection and identification of ANL-labeled proteins, significantly reducing the sample size, complexity, cost and time associated with BONCAT analysis.
Autores:
Aran, K.; Chooljian, M.; Paredes, J; et al.
Revista:
SCIENCE TRANSLATIONAL MEDICINE
ISSN:
1946-6234
Año:
2017
Vol.:
9
N°:
380
Noninvasive immunization technologies have the potential to revolutionize global health by providing easy-to-administer vaccines at low cost, enabling mass immunizations during pandemics. Existing technologies such as transdermal microneedles are costly, deliver drugs slowly, and cannot generate mucosal immunity, which is important for optimal immunity against pathogens. We present a needle-free microjet immunization device termedMucoJet, which is a three-dimensional microelectromechanical systems-based drug delivery technology. MucoJet is administered orally, placed adjacent to the buccal tissue within the oral cavity, and uses a self-contained gas-generating chemical reaction within its twocompartment plastic housing to produce a high-pressure liquid jet of vaccine. We show that the vaccine jet ejected from the MucoJet device is capable of penetrating the buccal mucosal layer in silico, in porcine buccal tissue ex vivo, and in rabbits in vivo. Rabbits treated with ovalbumin by MucoJet delivery have antibody titers of anti-ovalbumin immunoglobulins G and A in blood serum and buccal tissue, respectively, that are three orders of magnitude higher than rabbits receiving free ovalbumin delivered topically by a dropper in the buccal region. MucoJet has the potential to accelerate the development of noninvasive oral vaccines, given its ability to elicit antibody production that is detectable locally in the buccal tissue and systemically via the circulation.
Revista:
IEEE SENSORS JOURNAL
ISSN:
1530-437X
Año:
2016
Vol.:
16
N°:
7
Págs.:
1856 - 1864
Bacterial biofilms led to numerous problems in a wide variety of sectors as the medical environment, the food and water industry, or the naval sector. Completely developed biofilms are nearly impossible to eliminate due to the high antibiotic resistance these complex systems present. The lack of evidential indicators of their presence at the first stages of development makes antimicrobial treatments late and inadequate. Therefore, it is necessary to find new methods for the early detection of biofilm development in order to improve the efficiency of treatments by exposing bacterial cells before encapsulation in the extracellular matrix. For this purpose, this paper presents a real-time analysis of bacterial adhesion and biofilm growth by means of electrochemical measurements. Cyclic voltammetry and differential pulse voltammetry were performed with thin-film interdigitated microelectrode-based sensors. More sensitive and selective measurements were obtained with the second technique. Bacterial adhesion was detected 1 h after the initial inoculum, and three different redox centers were identified on bacterial surfaces. Finally, bacterial biofilm growth phases (lag, exponential, and stationary) were identified through the electrochemical measurements.
Autores:
Viswanathan, S.; Narayanan, T.; Aran, K.; et al.
Revista:
MATERIALS TODAY
ISSN:
1369-7021
Año:
2015
Vol.:
18
N°:
9
Págs.:
513 - 522
Chronic diseases are becoming more prevalent, and the complexities of managing patients continue to escalate, since their care must be balanced between the home and clinical settings. Diabetes is the most advanced example, where self-monitoring has been shown to be necessary. Glucometers are point-of-care (POC) devices that have become standard platforms at home and clinical settings. Similarly, many other POC biosensors have also been developed. Enzymes are often used in these sensors because of their specificity and the reaction products can be electrochemically transduced for the measurement. When enzymes are immobilized to an electronically active substrate, enzymatic reactions can be transduced by direct electron transport. This paper describes an approach for the development of graphene-based POC devices. This includes modifying enzymes for improved performance, developing methods to bind them to the graphene surface, incorporation of the functionalized graphene on a field-effect transistor (FET), and integration into a microfluidic device suitable for home use. This paper describes an approach for the development of a graphene-based POC biosensor platform using glucose as an example of target molecule.
Revista:
SENSORS AND ACTUATORS B-CHEMICAL
ISSN:
0925-4005
Año:
2015
Vol.:
216
Págs.:
263 - 270
Microfluidic devices play an increasingly important role in healthcare-related fields, but integration of electrodes and electronic components has been restricted at the prototyping stage of product development by a limited range of fabrication methods. In this work a new fabrication methodology is presented for embedding metallic microelectrodes in thermoplastic microfluidic devices. Microelectrodes are fabricated on steel wafers by means of photolithographic patterning and electrodeposition and then transferred to a thermoplastic sheet using hot embossing, resulting in embedded metal electrodes flush with the polymer surface. The unique shape of the microelectrodes provides an anchoring mechanism that ensures structural stability and reliability of the devices. A wide variety of thermoplastics can be used in this process including polycarbonate, polymethylmethacrylate (PMMA), and cyclic olefin copolymer (COC). Devices are assembled by a solvent-assisted bonding process, after drilling the inlets and outlets. This method allows for rapid fabrication of robust embedded electrodes and wiring connections from a broad range of metals for thermoplastic microfluidic devices. Finally, embedded interdigitated microelectrodes are used to measure conductivity within a microchannel via impedance spectroscopy analysis. The use of this technology is relevant to a wide range of analytical applications. (C) 2015 Elsevier B.V. All rights reserved.
Revista:
ADVANCED MATERIALS
ISSN:
0935-9648
Año:
2015
Vol.:
27
N°:
8
Págs.:
1433 - 1436
A digital point-of-care biosensor for measuring reactive oxygen species is presented based on novel reactive oxygen species responsive polymer-based electrodes. The biosensor is able to detect a drug-induced liver injury by monitoring the oxidative stress in the blood.
Revista:
SENSORS AND ACTUATORS B-CHEMICAL
ISSN:
0925-4005
Año:
2014
Vol.:
195
Págs.:
667 - 676
Bacterial biofilms are presented in many different environments causing a wide variety of infectious processes. Biofilms at their mature stage are difficult to eradicate because of their inherent resistance to antimicrobial agents. Easy-to-integrate and in situ detection tools would provide early detection of bacterial presence allowing efficient prophylactic actions. Impedance microbiology has been postulated as a suitable technique that allows monitoring of bacterial biofilm growths in real time. In this work four different culturing setups were developed as testing platforms for measuring real time microbiological cultures that could mimic real field environments. Results suggest that the position of the sensors in regard to the dynamic conditions of the culture might affect the sensitivity and the target parameter. Capacitance and resistance are associated to different biological effects, surface coating and conductivity changes respectively. Relative variations of electrical parameters were recorded in the lab obtaining significant changes in few hours post-infection. It has been proven that biological coating cause largest variations in capacitance, up to 60%, while metabolic activity affects more the resistance giving a variation up to 15%. Fitting analysis has confirmed experimental results showing also the effect of the dead/alive ratio. (C) 2014 Elsevier B.V. All rights reserved.
Revista:
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN:
0167-7012
Año:
2014
Vol.:
100
Págs.:
77 - 83
Impedance microbiology (IM) is a known technique that has been applied during the last decades to detect the presence of microorganisms in real samples in different fields: food industry, healthcare, environment, etc. Bacterial biofilms however have not been so far studied despite the fact that they are the most common microbiological formation and that they present resistance to antimicrobial agents. In situ early detection of bacterial biofilm is still a challenge nowadays that causes huge impact in many different scenarios. The ability to detect biofilm generation early will allow better and more efficient treatments preventing high costs and important problems. In this work a new performance of this technique with interdigitated microelectrode sensors (IDE) is proposed. A specific culturing setup where the sensors have been integrated in Petri Dishes has been developed. From the results it can be highlighted that low frequencies are more sensitive for detection than higher ones. The results achieved record variations of approximately 40% in the equivalent serial resistance after 10 h of culture. Electrical models have been successfully simulated to find the electrical behavior of the development of biofilms. Variations in both the capacitance and resistance were recorded during the growth of the microbes. (C) 2014 Elsevier B.V. All rights reserved.
Revista:
BIOMEDICAL MICRODEVICES
ISSN:
1387-2176
Año:
2014
Vol.:
16
N°:
3
Págs.:
365 - 374
Central venous catheters (CVC) are commonly used in clinical practice to improve a patient's quality of life. Unfortunately, there is an intrinsic risk of acquiring an infection related to microbial biofilm formation inside the catheter lumen. It has been estimated that 80 % of all human bacterial infections are biofilm-associated. Additionally, 50 % of all nosocomial infections are associated with indwelling devices. Bloodstream infections account for 30-40 % of all cases of severe sepsis and septic shock, and are major causes of morbidity and mortality. Diagnosis of bloodstream infections must be performed promptly so that adequate antimicrobial therapy can be started and patient outcome improved. An ideal diagnostic technology would identify the infecting organism(s) in a timely manner, so that appropriate pathogen-driven therapy could begin promptly. Unfortunately, despite the essential information it provides, blood culture, the gold standard, largely fails in this purpose because time is lost waiting for bacterial or fungal growth. This work presents a new design of a venous access port that allows the monitoring of the inner reservoir surface by means of an impedimetric biosensor. An ad-hoc electronic system was designed to manage the sensor and to allow communication with the external receiver. Historic data recorded and stored in the device was used as the reference value for the detection of bacterial biofilm. The RF communication system sends an alarm signal to the external receiver when a microbial colonization of the port occurs. The successful in vitro analysis of the biosensor, the electronics and the antenna of the new indwelling device prototype are shown. The experimental conditions were selected in each case as the closest to the clinical working conditions for the smart central venous catheter (SCVC) testing. The results of this work allow a new generation of this kind of device that could potentially provide more efficient treatments for catheter-related infections.
Revista:
SENSORS AND ACTUATORS B-CHEMICAL
ISSN:
0925-4005
Año:
2013
Vol.:
178
Págs.:
663 - 670
Bacterial biofilms are a common cause of persistent and chronic infections, mostly related to implantable devices. In this context, Staphylococcus species are the most relevant microorganisms involved in causing high costs for the healthcare system. New diagnostic and/or therapeutic tools should be developed by providing in vivo analysis of the specific physiological parameters directly related to the status of the indwelling device. Monitoring the biofilm's biological evolution will allow an earlier diagnostic. Impedance microbiology is suggested as a proper technique for directly measuring the development of bacterial biofilms generated inside intravascular catheters. In this study we propose interdigitated microelectrode biosensors be integrated within those implantable devices. In vitro assays have been carried out in order to characterize this methodology as a detection and monitoring tool for bacterial biofilm development. Impedance spectroscopy (IS) was implemented in 96-well microtiter plates, leading to positive results in detecting and monitoring bacterial biofilm development. Two Staphylococcus aureus and two Staphylococcus epidermidis strains were successfully monitored during a 20-h culture, and results show that the low range of the frequency is the most suitable setting for measuring the maximum relative changes. (c) 2013 Elsevier B.V. All rights reserved.
Revista:
BIOSENSORS AND BIOELECTRONICS
ISSN:
0956-5663
Año:
2012
Vol.:
38
N°:
1
Págs.:
226 - 232
Detection of device-associated infectious processes is still an important clinical challenge. Bacteria grow adhered to the device surfaces creating biofilms that are resistant to antimicrobial agents, increasing mortality and morbidity. Thus there is need of a surgical procedure to remove the indwelling infected device. The elevated cost of these procedures, besides patients discomfort and increased risks, highlights the need to develop more efficient, accurate and rapid detection methods. Biosensors integrated with implantable devices will provide an effective diagnostic tool. In vivo, rapid and sensitive detection of bacteria attached to the device surfaces will allow efficient treatments. Impedance spectroscopy technique would be an adequate tool to detect the adherence and the growth of the microorganism by monitoring the impedance characteristics. In this work a label-free interdigitated microelectrode (IDAM) biosensor has been developed to be integrated with implantable devices. Impedance characterization of Staphylococcus epidermidis biofilms has been performed achieving electrical monitoring of the bacterial growths in a few hours from the onset of the infection. This pathogen represents the most common microorganism related to intravascular catheters associated infections. The experimental setup presented in this work, a modified CDC biofilm reactor, simulates the natural environment conditions for bacterial biofilm development. The results prove that the low range of frequency is the most suitable setting for monitoring biofilm development. Our findings prove the effectiveness of this technique which shows variations of 59% in the equivalent serial capacitance component of the impedance. (C) 2012 Elsevier B.V. All rights reserved.
Nacionales y Regionales
Título:
Optimización de los parámetros de estimulación mecánica para el desarrollo y la maduración de orgánulos musculares in vitro en 3D. (OptiMUS)
Código de expediente:
PIBA_2022_1_0020
Investigador principal:
Jacobo Paredes Puente
Financiador:
GOBIERNO VASCO
Convocatoria:
PROYECTOS DE INVESTIGACIÓN BÁSICA Y APLICADA 2022-2024
Fecha de inicio:
11/08/2022
Fecha fin:
30/06/2025
Importe concedido:
50.000,00€
Otros fondos:
-
Título:
FRONTIERS 2021 - Superficies multifuncionales en la frontera del conocimiento
Código de expediente:
KK-2021/00124
Financiador:
GOBIERNO VASCO
Convocatoria:
2021 GV Elkartek -Proyectos de apoyo a la investigacion colaborativa en areas estratégicas.Tipo 1.
Fecha de inicio:
01/03/2021
Fecha fin:
31/12/2022
Importe concedido:
85.560,90€
Otros fondos:
-
Título:
Identificación de nuevas dianas para el tratamiento del glaucoma basados en los cambios biofísicos y presión intraocular sobre las HTMC en un modelo 3D de malla trabecular.
Código de expediente:
PI18/01782
Financiador:
INSTITUTO DE SALUD CARLOS III
Convocatoria:
AES2018 PI
Fecha de inicio:
01/01/2019
Fecha fin:
30/06/2023
Importe concedido:
154.880,00€
Otros fondos:
Fondos FEDER
