Detalle Publicación


Novel integrated and portable endotoxin detection system based on an electrochemical biosensor

Título de la revista: ANALYST
ISSN: 0003-2654
Volumen: 140
Número: 2
Páginas: 654 - 660
Fecha de publicación: 2015
This paper describes the design, implementation and validation of a sensitive and integral technology solution for endotoxin detection. The unified and portable platform is based on the electrochemical detection of endotoxins using a synthetic peptide immobilized on a thin-film biosensor. The work covers the fabrication of an optimized sensor, the biofunctionalization protocol and the design and implementation of the measuring and signalling elements (a microfluidic chamber and a portable potentiostat-galvanostat), framed ad hoc for this specific application. The use of thin-film technologies to fabricate the biosensing device and the application of simple immobilization and detection methods enable a rapid, easy and sensitive technique for in situ and real time LPS detection.