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Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue

Autores: Zapata Linares, Natalia María; Rodríguez Díaz, Saray; Mazo Vega, Manuel María; Abizanda Sarasa, Gloria María; Andreu Oltra, Enrique José; Barajas Vélez, Miguel Ángel; Prosper Cardoso, Felipe; Rodríguez Madoz, Juan Roberto
Título de la revista: STEM CELL RESEARCH
ISSN: 1873-5061
Volumen: 16
Número: 1
Páginas: 20 - 23
Fecha de publicación: 2016
Resumen:
In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.
Enlace DADUN: http://hdl.handle.net/10171/39951
DOI: https://doi.org/10.1016/j.scr.2015.12.002
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