Our researchers

María del Carmen Antolín Bellver

Research lines
Fisiología Vegetal
H-Index
20, (WoS, 06/12/2016)

Most recent scientific publications (since 2010)

Authors: Goicoechea, María Nieves; Antolín, María del Carmen;
Journal: MICROBIAL BIOTECHNOLOGY
ISSN 1751-7907  Vol. 10  Nº 5  2017  pp. 1004 - 1007
Modern agriculture and horticulture must combine two objectives that seem to be almost mutually exclusive: to satisfy the nutritional needs of an increasing human population and to minimize the negative impact on the environment. These two objectives are included in the Goal 2 of the 2030 Agenda for Sustainable Development of the United Nations: End hunger, achieve food security and improved nutrition and promote sustainable agriculture'. Enhancing the nutritional levels of vegetables would improve nutrient intake without requiring an increase in consumption. In this context, the use of beneficial rhizospheric microorganisms for improving, not only growth and yield, but also the nutrient quality of crops represents a promising tool that may respond to the challenges for modern agriculture and horticulture and represents an alternative to the genetic engineering of crops. This paper summarizes the state of the art, the current difficulties associated to the use of rhizospheric microorganisms as enhancers of the nutritional quality of food crops as well as the future prospects.
Authors: N.; G.; J.; et al.
Journal: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN 0889-1575  Vol. 62  2017  pp. 51 - 62
Throughout the southern Mediterranean regions of Europe, projected climate warming combined with severe droughts during the growing season may alter grape metabolism, thus modifying the nutritional value of berries and the quality of wines. This study investigated the effects of pre- and post-veraison drought under elevated temperatures on berry skin metabolism of two Tempranillo clones (CL). Experimental assays were performed on fruit-bearing cuttings from CL-1089 and CL-843 of Vitis vinifera (L.) cv. Tempranillo subjected to two temperature regimes (24/14 °C or 28/18 °C (day/night)) combined with three irrigation regimes during berry ripening: (i) water deficit from fruit set to veraison (early deficit, ED); (ii) water deficit from veraison to maturity (late deficit, LD); and (iii) full irrigation (FI). At 24/14 °C, the LD treatment performed better than the ED treatment. Differences were attenuated at 28/18 °C and responses were modulated by type of clone. Elevated temperatures induced the accumulation of hexoses and amino acids in berries. ED at 24/14 °C reduced anthocyanins and flavonols, which may decrease the antioxidant properties of fruits. In contrast, the levels of these secondary metabolites did not decrease when LD was applied. Our results suggest that the adaptation of grapevines for climate change might be plausible with the optimization of timing of water deficit and the appropriate selection of clones.
Authors: Antolín, María del Carmen; I.; Goicoechea, María Nieves; et al.
Journal: THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY
ISSN 2197-0025  Vol. 28  Nº 2  2016  pp. 171 - 191
This paper describes the use of fruit-bearing grapevine hardwood cuttings as a model system for grapevine research, translating some studies that are difficult to execute under field conditions in the vineyards to facilities under controlled conditions. This approach enables to simulate in greenhouses future climate conditions and to investigate putative responses of grapevine to climate change. An updated description of how to grow grapevine fruit-bearing cuttings is made, together with modifications to carry out studies of partial rootzone drying, regulated deficit irrigation studies and symbiosis with arbuscular mycorrhizal fungi. We summarize how extensive has been the use of fruit-bearing cuttings in grapevine research over the years, with special emphasis in those experiments that analyze the effects of factors related to climate change, such as elevated CO2, elevated temperature, water availability and UV-B radiation, on grapevine physiology, production and grape quality. A validation of the model is made, comparing results obtained with fruit-bearing cuttings with those obtained from vineyard-grown plants. We discuss some advantages of growing grapevines under elevated CO2 with an atmosphere depleted in C-13, using this stable isotope (C-13) and others (N-15, Fe-54 or Fe-57, etc.) as tracers for C, N and other nutrient metabolism studies.
Authors: Torres, N.; Goicoechea, María Nieves; Antolín, María del Carmen;
Journal: CROP AND PASTURE SCIENCE
ISSN 1836-0947  Vol. 67  Nº 9  2016  pp. 961 - 977
The projected increase in mean temperatures caused by climate change is expected to have detrimental impacts on berry quality. Microorganisms as arbuscular mycorrhizal fungi (AMF) produce numerous benefits to host plants and can help plants to cope with abiotic stresses such as high temperature. The aims of this research were to characterise the response of three clones of Vitis vinifera L. cv. Tempranillo to elevated temperatures and to determine whether AMF inoculation can improve berry antioxidant properties under these conditions. The study was carried out on three fruit-bearing cuttings clones of cv. Tempranillo (CL-260, CL-1048 and CL-1089) inoculated with AMF or uninoculated and subjected to two temperature regimes (day¿night: 24°C-14°C and 28°C-18°C) during berry ripening. Results showed that clonal diversity of Tempranillo resulted in different abilities to respond to elevated temperature and AMF inoculation. In CL-1048, AMF inoculation improved parameters related to phenolic maturity such as anthocyanin content and increased antioxidant activity under elevated temperature, demonstrating a protective role of AMF inoculation against warming effects on berry quality. The results therefore suggest that selection of new clones and/or the implementation of measures to promote the association of grapevines with AMF could be strategies to improve berry antioxidant properties under future warming conditions.
Authors: Torres Molina, N.; Goicoechea, María Nieves; Antolín, María del Carmen;
Journal: INDUSTRIAL CROPS AND PRODUCTS
ISSN 0926-6690  Vol. 76  2015  pp. 77 - 85
Within climate change scenario, the maintenance of grape quality and wine characteristics will be the main concern for viticulture in the future years. However, changes in the composition of grapevine pruning wastes (i.e., leaves and stems) could be another interesting aspect as important antioxidant source for pharmaceutical industry due its richness in phenolic compounds beneficial for human health. To date, the effect of biotic and environmental factors in the accumulation of these compounds in leaves had received little attention. Therefore, the aims of study were (1) to evaluate the effect of biotic (mycorrhizal inoculation) and environmental (temperature) factors, alone or combined, on phenolic composition and antioxidant activity of leaf extracts of grapevine and (2) to determine whether such effects differed among accessions of the same cultivar of grapevine. The study was carried out using container-grown grapevines grown in greenhouses. Dormant Vitis vinifera (L.) cuttings of different accessions of Tempranillo were selected to get fruit-bearing cuttings. At transplanting, half of the plants of each accession were inoculated with the mycorrhizal inoculum and after fruit set, plants were exposed to two temperature regimes (24. °C/14. °C and 28. °C/18. °C (day/night)) to commercially berry ripe. Results showed that total phenolic content, antioxidant compounds like flavonols and anthocyanins, and antioxidant activity of leaves were improved with mycorrhizal inoculation under high temperature conditions. It was concluded that mycorrhizal inoculation of grapevines could contribute to preserve high level of antioxidant compounds of leaves in a future climate change scenario. However, the effects were strongly dependent of accession assayed, which indicated a significant intra-varietal diversity in the response of Tempranillo to biotic and environmental factors.
Authors: Antolín, María del Carmen; Lovato, M. E.; et al.
Journal: SCIENTIA HORTICULTURAE
ISSN 0304-4238  Vol. 193  2015  pp. 359 - 366
The aim of this study was to analyze the impact of different doses of compost obtained from fats waste of dairy industry mixed with commercial peat-based substrate and with soil on pepper plants development. Furthermore, this investigation attempted to determine the effects of these mixtures on germination and composition of pepper fruits. Fat compost (FC) was obtained by aerobic composting from dairy industry wastewaters mixed with wood chips and green grass. FC extract resulted free of phytotoxicity for seed germination, obtained 99.71% the relative seed germination and 74.10% of germination index. Different growing media were prepared by mixing 0, 10, 20 and 40% of FC with commercial substrate (CS) or Soil in two different experiments. Results showed that the application of FC on CS and Soil respectively, increased plant dry matter. On pepper seedling FC 40 achieves 250mg plant-1 DM. Moreover, higher doses of FC improved yield and several characteristics of fruit as dry matter, diameter and concentrations of some carotenoids. The incorporation of FC did not increase the heavy metals concentration of pepper fruit. At leaves, the highest concentrations of N were reached shown on treatment with FC (Soil: 1.46%, FC 10: 1.92%, FC 20: 2.00%, FC 40: 2.09%). Application of FC for germination and development of pepper plants improved the seedlings, fruit yield and quality.
Authors: Fiasconaro, M. L.; Antolín, María del Carmen; Lovato, M. E.; et al.
Journal: SCIENTIA HORTICULTURAE
ISSN 0304-4238  Vol. 193  2015  pp. 359 - 366
The aim of this study was to analyze the impact of different doses of compost obtained from fats waste of dairy industry mixed with commercial peat-based substrate and with soil on pepper plants development. Furthermore, this investigation attempted to determine the effects of these mixtures on germination and composition of pepper fruits. Fat compost (FC) was obtained by aerobic composting from dairy industry wastewaters mixed with wood chips and green grass. FC extract resulted free of phytotoxicity for seed germination, obtained 99.71% the relative seed germination and 74.10% of germination index. Different growing media were prepared by mixing 0, 10, 20 and 40% of FC with commercial substrate (CS) or Soil in two different experiments. Results showed that the application of FC on CS and Soil respectively, increased plant dry matter. On pepper seedling FC 40 achieves 250 mg plant(-1) DM. Moreover, higher doses of FC improved yield and several characteristics of fruit as dry matter, diameter and concentrations of some carotenoids. The incorporation of FC did not increase the heavy metals concentration of pepper fruit. At leaves, the highest concentrations of N were reached shown on treatment with FC (Soil: 1.46%, FC 10: 1.92%, FC 20: 2.00%, FC 40: 2.09%). Application of FC for germination and development of pepper plants improved the seedlings, fruit yield and quality.
Authors: Niculcea, Maria; L.; Z.; et al.
Journal: VITIS
ISSN 0042-7500  Vol. 54  Nº 1  2015  pp. 9 - 16
The response of phenolic composition of skins from Vitis vinifera L. 'Tempranillo' and 'Graciano' grapes to water-deficit irrigation during berry growth and ripening was evaluated. The study was carried out using container-grown grapevines grown under controlled conditions in a greenhouse. Two irrigation treatments were imposed: control (well-watered) and sustained deficit irrigation (SDI). Twenty-eight phenolic compounds, including anthocyanins, flavonols and monomeric flavan-3-ols (catechins) as well as phenolic acids derivatives have been identified in the extracts prepared from the berry skins at physiological maturity. For both varieties, water deficit reduced leaf area and leaf area to crop mass ratio, and decreased berry size. However, there were no changes in juice total soluble solids, pH or total polyphenolic content. Water deficit resulted in decreased must titratable acidity in 'Graciano' berries. In 'Tempranillo', water limitation reduced total anthocyanins and flavonols, and increased hydroxycinnamic acids. In 'Graciano', water deficit resulted in increased flavonols and reduced catechins. Altogether, we concluded that under water-deficit irrigation, 'Graciano' grapes presented a differential composition of phenolic compounds that could result in improved fruit quality.
Authors: Pascual, I; Aguirreolea, Jone Miren; et al.
Journal: PLANT SCIENCE
ISSN 0168-9452  Vol. 226  2014  pp. 30 - 40
Human activities are increasing atmospheric CO2 concentration and temperature. Related to this global warming, periods of low water availability are also expected to increase. Thus, CO2 concentration, temperature and water availability are three of the main factors related to climate change that potentially may influence crops and ecosystems. In this report, we describe the use of growth chamber - greenhouses (GCG) and temperature gradient greenhouses (TGG) to simulate climate change scenarios and to investigate possible plant responses. In the GCG, CO2 concentration, temperature and water availability are set to act simultaneously, enabling comparison of a current situation with a future one. Other characteristics of the GCG are a relative large space of work, fine control of the relative humidity, plant fertirrigation and the possibility of light supplementation, within the photosynthetic active radiation (PAR) region and/or with ultraviolet-B (UV-B) light. In the TGG, the three above-mentioned factors can act independently or in interaction, enabling more mechanistic studies aimed to elucidate the limiting factor(s) responsible for a given plant response. Examples of experiments, including some aimed to study photosynthetic acclimation, a phenomenon that leads to decreased photosynthetic capacity under long-term exposures to elevated CO2, using GCG and TGG are reported.
Authors: Niculcea, Maria; J.; et al.
Journal: AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
ISSN 1322-7130  Vol. 20  Nº 2  2014  pp. 281 - 291
Background and Aims The application of deficit irrigation to grapevines modifies the hormonal status of berries, but little information about the influence of berry hormones on phenological sensitivity to water deficit is available. Therefore, the aim of this research was to assess the involvement of berry hormonal status in fruit composition in response to regulated deficit irrigation applied during different phenological stages in two grapevine cultivars. Methods and Results The study was carried out on fruiting cuttings of two cultivars of Vitis viniferaL., Tempranillo and Graciano. Treatments were: (i) early water deficit from fruitset to onset of veraison (early deficit); (ii) late water deficit from onset of veraison to harvest (late deficit); and (iii) plants regularly irrigated (Control). Both early water deficit and late water-deficit strategies modified evolution of indole-3-acetic acid, abscisic acid, salicylic acid and jasmonic acid, which was related to changes in berry size, increases in phenolic substances and accumulation of amines. Conclusions Differential sensitivity of Tempranillo and Graciano grapevines to seasonal water stress was mediated, at least in part, by alterations in hormonal status of berries at the time of water stress imposition. Significance of Study This study relates interspecific differences in the sensitivity of seasonal water-deficit irrigation to changes in the endogenous hormonal status of berries.
Authors: Baslam, Marouane; Antolín, María del Carmen; Gogorcena, Y.; et al.
Journal: ANNALS OF APPLIED BIOLOGY
ISSN 0003-4746  Vol. 164   Nº 2  2014  pp. 190 - 199
Alfalfa is a widely distributed forage legume whose leaves are high in protein content and whose stems are suitable for bioethanol production. However, alfalfa forage digestibility, quality and yield may vary under future climate change scenarios. This legume can establish double symbiosis with nitrogen-fixing bacteria and arbuscular mycorrhizal fungi (AMF). The presence of AMF can modify the evolution of biomass production and partitioning during the vegetative growth of alfalfa. We hypothesised that mycorrhizal symbiosis may change the quantity and/or quality of carbohydrates and lignin in leaves and/or stems of alfalfa, with these changes being dependent on the atmospheric CO2 concentration at which plants are grown. Results showed that mycorrhizal alfalfa plants exposed to elevated CO2 had improved leaf, stem and root biomass, enhanced amount of hemicellulose and decreased concentration of lignin in cell walls of leaves as well as increased levels of glucose and fructose in stems compared with non-mycorrhizal alfalfa. These results indicated improved forage quality (leaves) and enhanced potential for bioethanol conversion (stems) in mycorrhizal alfalfa cultivated under elevated CO2. Moreover, the potential of stems for producing CH4 reinforced their suitability for the conversion of biomass into bioethanol.
Authors: Irigoyen, Juan José; Goicoechea, María Nieves; Antolín, María del Carmen; et al.
Journal: PLANT SCIENCE
ISSN 0168-9452  Vol. 226  2014  pp. 22 - 29
Continued emissions of CO2, derived from human activities, increase atmospheric CO2 concentration. The CO2 rise stimulates plant growth and affects yield quality. Effects of elevated CO2 on legume quality depend on interactions with N2-fixing bacteria and mycorrhizal fungi. Growth at elevated CO2 increases photosynthesis under short-term exposures in C3 species. Under long-term exposures, however, plants generally acclimate to elevated CO2 decreasing their photosynthetic capacity. An updated survey of the literature indicates that a key factor, perhaps the most important, that characteristically influences this phenomenon, its occurrence and extent, is the plant source-sink balance. In legumes, the ability of exchanging C for N at nodule level with the N2-fixing symbionts creates an extra C sink that avoids the occurrence of photosynthetic acclimation. Arbuscular mycorrhizal fungi colonizing roots may also result in increased C sink, preventing photosynthetic acclimation. Defoliation (Anthyllis vulneraria, simulated grazing) or shoot cutting (alfalfa, usual management as forage) largely increases root/shoot ratio. During re-growth at elevated CO2, new shoots growth and nodule respiration function as strong C sinks that counteracts photosynthetic acclimation. In the presence of some limiting factor, the legumes response to elevated CO2 is weakened showing photosynthetic acclimation. This survey has identified limiting factors that include an insufficient N supply from bacterial strains, nutrient-poor soils, low P supply, excess temperature affecting photosynthesis and/or nodule activity, a genetically determined low nodulation capacity, an inability of species or varieties to increase growth (and therefore C sink) at elevated CO2 and a plant phenological state or season when plant growth is stopped.
Authors: Antolín, María del Carmen;
Journal: JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
ISSN 1436-8730  Vol. 176  Nº 1  2013  pp. 110 - 117
Leguminous plants grown in sewage sludge-amended soils can acquire nitrogen by assimilation of nitrate and ammonium from the soil solution or from atmospheric-dinitrogen (N-2) fixation through association with N-2-fixing bacteria. We proposed that operation of both metabolic processes could contribute to alleviate the impact of drought in sludge-treated plants. A greenhouse experiment was conducted to evaluate the involvement of nodule metabolism in the use efficiency of water and N in sludge-treated plants. Treatments comprised (1) plants inoculated with rhizobia and amended with sewage sludge; (2) plants inoculated with rhizobia without any amendment; and (3) noninoculated plants supplied with ammonium nitrate, each under well-watered and drought conditions. Under drought, sludge-treated plants had increased plant growth and higher photosynthetic and water-use efficiencies than untreated plants. Drought stimulated nitrate reductase and GS/GOGAT activities but did not affect the activities of phosphoenolpyruvate carboxylase and malate dehydrogenase or the leghemoglobin concentration. The results suggest that under drought conditions, both N-2 fixation and nitrate assimilation in nodules of sludge-treated plants contributed to improve plant N supply and to increase the drought tolerance of alfalfa.
Authors: P.; R.; et al.
Journal: PLANT AND CELL PHYSIOLOGY
ISSN 0032-0781  Vol. 54  Nº 7  2013  pp. 1200 - 1216
Berry organoleptic properties are highly influenced by ripening environmental conditions. In this study, we used grapevine fruiting cuttings to follow berry ripening under different controlled conditions of temperature and irradiation intensity. Berries ripened at higher temperatures showed reduced anthocyanin accumulation and hastened ripening, leading to a characteristic drop in malic acid and total acidity. The GrapeGen GeneChip (R) combined with a newly developed GrapeGen 12Xv1 MapMan version were utilized for the functional analysis of berry transcriptomic differences after 2 week treatments from veraison onset. These analyses revealed the establishment of a thermotolerance response in berries under high temperatures marked by the induction of heat shock protein (HSP) chaperones and the repression of transmembrane transporter-encoding transcripts. The thermotolerance response was coincident with up-regulation of ERF subfamily transcription factors and increased ABA levels, suggesting their participation in the maintenance of the acclimation response. Lower expression of amino acid transporter-encoding transcripts at high temperature correlated with balanced amino acid content, suggesting a transcriptional compensation of temperature effects on protein and membrane stability to allow for completion of berry ripening. In contrast, the lower accumulation of anthocyanins and higher malate metabolization measured under high temperature might partly result from imbalance in the expression and function of their specific transmembrane transporters and expression changes in genes involved in their metabolic pathways. These results open up new views to improve our understanding of berry ripening under high temperatures.
Authors: Niculcea, Maria; L.; Z.; et al.
Journal: JOURNAL OF PLANT GROWTH REGULATION
ISSN 0721-7595  Vol. 32  Nº 3  2013  pp. 551 - 563
Water-deficit irrigation to grapevines reduces plant growth, yield, and berry growth, altering the ripening process, all of which may influence fruit composition and wine quality. Therefore, the goals of this study were (1) to investigate the influence of the main endogenous berry hormones, abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acid (JA), on berry growth and ripening under water-deficit conditions and (2) to analyze changes in fruit composition, specifically N compounds, under water deprivation. The study was carried out using container-grown Tempranillo grapevines grown under controlled conditions in a greenhouse. Two irrigation treatments were imposed: control (well-watered) and sustained deficit irrigation (SDI). Water deficit decreased leaf area and the source-to-sink ratio, reduced yield and berry size, and decreased concentrations of the main phenolic compounds. SDI also modified berry hormonal status. At the pea-size stage, SDI berries had lower IAA and higher JA and SA than nonstressed berries. At veraison (onset of ripening), accumulation of ABA was less accentuated in SDI than in control berries. At harvest, the content of amino acids and free ammonium was low in both treatments but SDI-treated berries showed a significant accumulation of amines. Results suggest that water restrictions to grapevines might be playing a physiological role in reducing berry growth through affecting hormone dynamics, phenolic synthesis, and the berry amino acid content and composition, which could compromise fruit quality. Possible roles of endogenous IAA controlling berry size and endogenous ABA and SA controlling levels of anthocyanins and flavonols at harvest are discussed.
Authors: Gogorcena, Y.; Muñoz, F.; et al.
Journal: Plant Science
ISSN 0168-9452  Vol. 191  2012  pp. 16 - 23
Symbiotic association of legumes with rhizobia frequently results in higher photosynthesis and soluble carbohydrates in comparison with nitrate-fed plants, which might improve its potential for biomass conversion into bioethanol. A greenhouse experiment was conducted to examine the effects of nitrogen source and water availability on stem characteristics and on relationships between carbohydrates, phenolic metabolism activity and cell wall composition in alfalfa (Medicago sativa L cv. Aragon). The experiment included three treatments: (1) plants fed with ammonium nitrate (AN): (2) plants inoculated with rhizobia (R); and (3) plants inoculated with rhizobia and amended with sewage sludge (RS). Two levels of irrigation were imposed: (1) well-watered and (2) drought stress. Under well-watered conditions, nitrogen-fixing plants have increased photosynthesis and stem fermentable carbohydrate concentrations, which result in higher potential for biomass conversion to bioethanol than in AN plants. The latter had higher lignin due to enhanced activities of phenolic metabolism-related enzymes. Under drought conditions, the potential for bioethanol conversion decreased to a similar level in all treatments. Drought-stressed nitrogen-fixing plants have high concentrations of fermentable carbohydrates and cell wall cellulose, but ammonium nitrate-fed plants produced higher plant and stem biomass, which might compensate the decreasing stem carbohydrates and cellulose concentrations.
Authors: Antolín, María del Carmen;
Journal: Ecotoxicology and Environmental Safety
ISSN 0147-6513  Vol. 73  Nº 3  2010  pp. 436 - 442
Authors: Antolín, María del Carmen;
Journal: Environmental and Experimental Botany
ISSN 0098-8472  Vol. 68  Nº 1  2010  pp. 75 - 82
Authors: Antolín, María del Carmen;
Journal: Journal of Hazardous Materials
ISSN 0304-3894  Vol. 182  Nº 1-3  2010  pp. 210 - 216
Authors: Antolín, María del Carmen; Ayari, Marouen; et al.
Book title:  Methodologies and results in grapevine research
2010  pp. 163-151
Authors: Antolín, María del Carmen, (Editor)
2016 

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  Other relevant merits

he is a PhD in Biological Sciences from 1991 and Professor of Plant Physiology since 1992. She has the positive accreditation from ANECA (National Agency for Assessment and Accreditation, Spain) as Associate Professor since 2003 and as Assistant Professor since 2008. She is a researcher member of the Associated Unit of the CSIC (Superior Council for Scientific Research of Spain) formed among the Department of Plant Biology (Plant Biology Section) from the University of Navarra, the Experimental Station of Aula Dei (Zaragoza) and the Institute of Sciences of the Vine and Wine (ICVV) (Logroño) from the start (2006 and 2008 respectively ). She is the author of 45 articles in scientific journals indexed with an index of relative quality (70% Q1) and 10 book chapters. She has participated in 75 conferences both nationally and internationally as well as 22 research projects. She has also directed 9 doctoral these and other research. She currently directs a doctoral thesis and participates in 2 projects funded research by national and European organizations. She is a reviewer for 30 international scientific journals. The research lines in which she is specialized are Grapevine physiology, plant responses to abiotic and biotic stresses, organic waste management and agricultural application of sewage sludge. In addition, it is reviewing several articles in international journals and has 3 six-year research recognized by CNEAI (National Evaluation Commission of Research Activity, Spain).